Up to present, there are only few scientific publications on anthropogenic fibers in atmospheric fallout. There is no well-documented protocol for isolation the fibers from the samples. In this study, two approaches for the isolation of anthropogenic fibers have been proposed. Protocol 1 is just done with only filtration step, without any treatment procedure. Protocol 2 includes several treatment steps before filtration (SDS, Biozyms SE & F, H2O2). Two Protocols have been conducted with atmospheric fallout samples taken in an urban location of Ho Chi Minh City. The filters from two approaches are then observed using a stereomicroscope. The stereomicroscopic observation shows that Protocol 2 effectively work for these samples.