Nghiên cứu sự tái sinh một bước in vitro của giống mía KK3 (Saccharum officinarum L.)

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Tác giả: Thị Thu Hiền Phan

Ngôn ngữ: Vie

Ký hiệu phân loại:

Thông tin xuất bản: Khoa học & công nghệ Việt Nam 2022

Mô tả vật lý: 70 - 74

Bộ sưu tập: Metadata

ID: 406394

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Nghiên cứu đã thiết lập được công thức môi trường tái sinh trực tiếp của giống mía KK3. Cụ thể, môi trường thích hợp nhất cho việc tái sinh trực tiếp từ cuộn lá non giống mía KK3 là RE2 (MS + 5 mg/l α-NAA + 1 mg/l Kinetin), tỷ lệ tái sinh đạt 25,27% và số chồi hình thành/1 g cuộn lá non là 11,56. Nghiên cứu cho thấy, vị trí mảnh cắt cuộn lá non phù hợp cho tái sinh trực tiếp nhất là cách đỉnh sinh trưởng 2-6 cm, tỷ lệ tái sinh chồi đạt 25,40%, mẫu tái sinh tốt, tỷ lệ tạo đa chồi cao. Khi sử dụng mảnh cắt thực vật có độ dày 1 cm, tỷ lệ tái sinh đạt cao nhất 25,58%, khả năng hình thành đa chồi cao. Thời gian tiền nuôi cấy có ảnh hưởng tích cực lên khả năng tái sinh chồi trực tiếp từ cuộn lá non của giống mía KK3. Khi tiến hành tiền nuôi cấy trong thời gian 4 ngày giúp tăng khả năng tái sinh của giống mía KK3 (đạt 47,49%). Môi trường MS + 5 mg/l α-NAA + 1 mg/l Kinetin có bổ sung 30% nước dừa cho thấy khả năng tái sinh cao nhất (52,95%) và đạt trung bình 52,33 chồi/1 g cuộn lá non., Tóm tắt tiếng anh, The research has successfully created the formula for direct regeneration medium of sugarcane variety KK3. In detail, the most suitable medium for direct regeneration from young leaf rolls of sugarcane variety KK3 was RE2 (MS + 5 mg/l α-NAA + 1 mg/l Kinetin), the regeneration rate was 25.27%, the number of shoots formed/1.0 g of young leaf rolls reached 11.56 shoots. The study showed that the position of the young leaf rolls cuttings suitable for direct regeneration was the cuttings located from 2-6 cm away from the growth apex. The shoot regeneration rate reached 25.40%, the regenerating samples were 25.40%, good yield, and a high rate of multiple shoot formation. When using plant cuttings with a thickness of 1 cm, the highest regeneration rate was 25.58%, and the ability to form multiple shoots was high. Pre-cultivation time had a positive effect on the ability to regenerate shoots directly from young leaf coils of sugarcane variety KK3. When conducting pre-cultivation in four days will help increase the regeneration ability of the sugarcane variety, reaching 47.49%. MS medium + 5 mg/l α-NAA + 1 mg/l Kinetin supplemented with 30% coconut water showed the highest regeneration ability, reaching 52.95%, number of shoots reaching 52.33 shoots/1 g of young leaf rolls.

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1. Đọc trực tuyến

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