Đặc điểm của các dòng lợi khuẩn Bacillus spp. từ tôm thẻ chân trắng (Litopenaeus vannamei) ở tỉnh Kiên Giang

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Tác giả: Ngọc Thanh Tâm Huỳnh, Văn Thịnh Huỳnh

Ngôn ngữ: Vie

Ký hiệu phân loại: 639 Hunting, fishing, conservation, related technologies

Thông tin xuất bản: Tạp chí Khoa học Đại học Cần Thơ, 2020

Mô tả vật lý: 44-52

Bộ sưu tập: Metadata

ID: 407097

Thêm vào giỏ Liên kết toàn văn

Nghiên cứu được thực hiện nhằm phân lập những dòng vi khuẩn Bacillus spp. có những đặc tính phù hợp để sản xuất chế phẩm sinh học nhằm kiểm soát các tác nhân gây bệnh. Từ hệ tiêu hóa của các mẫu tôm thẻ chân trắng thu từ ao nuôi thuộc địa bàn tỉnh Kiên Giang đã phân lập được 20 dòng vi khuẩn Bacillus spp. Kết quả có 11 trên tổng số 20 dòng thể hiện khả năng đối kháng với ít nhất 1 dòng vi khuẩn kiểm định gồm Escherichia coli, Staphyloccocus aureus, Aeromonas sp. và Pseudomonas sp. Kết quả cũng cho thấy cả 11 dòng vi khuẩn đều có khả năng sinh ít nhất 2 loại enzyme ngoại bào trong nhóm amylase, cellulase và protease. Dòng AM2 và AM3 được lựa chọn để khảo sát khả năng chịu mặn và chịu pH, kết quả cho thấy, cả 2 dòng đều có khả năng phát triển trong môi trường được bổ sung nồng độ muối từ 1 - 5% và pH từ 4 - 9, dòng AM2 và AM3 tương đồng 99% với dòng Bacillus subtilis và Bacillus cereus dựa trên kết quả giải trình tự 16S rRNA đã được đăng ký trên GenBank với mã số lần lượt là MN907469 và MN907471., Tóm tắt tiếng anh, This study is aimed to isolate beneficial Bacillus spp. strains for producing biological products to control pathogens. Twenty Bacillus spp. strains was isolated from digestive system of white leg shrimps (Litopenaeus vannamei) collected from shrimp ponds in Kien Giang province. The results show that 11 isolates acted as an antagonist of at least one of pathogenic strains including Escherichia coli, Staphylococcus aureus, Aeromonas sp., and Pseudomonas sp. Eleven isolates have the potential production at least two extracellular enzymes including amylase, cellulase and protease. The AM2 and AM3 were selected to examine salinity and pH tolerance ability, and both strains represented their abilities to grow at the pH ranges from 4 to 9 and the salinity ranges from 1 to 5%. The results of 16S rRNA genotype sequencing for the two strains, AM2 and AM3 indicated that the AM2 sequence was homologous up to 99% with B. sutilis, and AM3 strain sequence was homologous up to 99% with Bacillus cereus and registered on GenBank with the code consecutively MN907469 and MN907471.

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