Các hợp chất saponin khung dammarane từ rễ cây tam thất

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Tác giả: Văn Hùng Hoàng, Quang Tấn Lục

Ngôn ngữ: Vie

Ký hiệu phân loại: 633 Field and plantation crops

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ Đại học Thái Nguyên, 2022

Mô tả vật lý: 45362

Bộ sưu tập: Metadata

ID: 407605

Thêm vào giỏ Liên kết toàn văn

Từ dịch chiết methanol của rễ cây tam thất (Panax pseudoginseng), sáu hợp chất saponin có khung dammarane đã được phân lập. Các hợp chất được xác định là (20S)-ginsenoside Rh1 (1), (20R)-ginsenoside Rh1 (2), ginsenoside Rk3 (3), 6-acetoxy-ginsenoside Rh1 (4), ginsenoside F1 (5), và ginsenoside Rg3 (6) bằng phân tích các phổ khối lượng phân giải cao (HR-ESI-MS), phổ cộng hưởng từ hạt nhân một chiều (1D NMR) và hai chiều (2D NMR), kết hợp so sánh với các dữ liệu đã được công bố. Kết quả đánh giá sàng lọc hoạt tính ức chế sản sinh NO đối với tế bào RAW264,7 kích thích bởi LPS cho thấy cả 6 hợp chất này đều có hoạt tính khá tốt. Hợp chất 1-6 thể hiện hoạt tính ức chế sản sinh NO trong các tế bào RAW 264,7 được kích thích bởi LPS với giá trị IC50 = 17,32 ± 1,56 ~ 34,61 ± 2,72 µM, so với giá trị tương ứng của chất đối chứng NG-monomethyl-L-arginine (L-NMMA), IC50 = 9,80 ± 0,78 µM.Kết quả này giúp làm rõ thêm về thành phần hóa học của rễ cây tam thất ở Việt Nam. Hơn nữa, kết quả sàng lọc hoạt tính trên gợi mở cho những nghiên cứu tiếp theo đối với các sản phẩm kháng viêm và tăng cường khả năng miễn dịch của rễ cây tam thất., Tóm tắt tiếng anh, From the methanol extract of the roots of Panax pseudoginseng, six saponins compounds with dammarane frame were isolated. The compounds including (20S)-ginsenoside Rh1 (1), (20R)-ginsenoside Rh1 (2), ginsenoside Rk3 (3), 6-acetoxy-ginsenoside Rh1 (4), ginsenoside F1 (5), and ginsenoside Rg3 (6) were identified by high- resolution mass spectrometry analysis (HR-ESI-MS), one-dimensional (1D NMR) and two-dimensional (2D NMR) nuclear magnetic resonance spectroscopy, combined with comparison of existing data.The results of screening evaluation of NO production inhibitory activity against RAW264.7 cells stimulated by LPS showed that all 6 compounds had quite good activity. Compound 1-6 exhibited inhibitory activity on NO production in LPS-stimulated RAW 264.7 cells with IC50 value = 17.32 ± 1.56 ~ 34.61 ± 2.72 µM, compared with the corresponding value of NG-monomethyl-L-arginine (L-NMMA) control, IC50 = 9.80 ± 0.78 µM. This result helps to clarify more about the chemical composition of the Panax pseudoginseng plant in Vietnam. Furthermore, the results of analysis suggest for further studies on anti-inflammatory and immune-enhancing properties from the roots of Panax pseudoginseng.

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