PROPAGATION OF ME LINH ROSE BY PLANT TISSUE CULTURE

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Tác giả: Đức Hà Chu, Việt Hồng La, Thị Quỳnh Ngô

Ngôn ngữ: Vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ Đại học Thái Nguyên, 2019

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ID: 408279

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Cây hoa hồng là một trong những loại hoa cắt cành quan trọng nhất trên thế giới và ở Việt Nam hiện nay. Trong nghiên cứu này, hoa hồng Mê Linh (Hà Nội), đã được sử dụng làm vật liệu cho nhân giống in vitro phục vụ nguồn cung ứng cây sạch bệnh. Kết quả cho thấy, tất cả các công thức có bổ sung BAP đều kích thích hình thành chồi và môi trường tốt nhất để tái sinh chồi in vitro từ đốt thân hoa hồng là MS có bổ sung 1,0 mg/l BAP, chồi tái sinh phát triển tốt. Công thức môi trường thích hợp cho nhân nhanh chồi in vitro được xác định là MS bổ sung 1,0 mg/l BAP, số lượng chồi/mẫu đạt 3,80
chiều cao chồi đạt 1,70 cm và số lá/chồi đạt 4,20. Chiều cao chồi lớn nhất được ghi nhận ở công thức có bổ sung 0,2 mg/l than hoạt tính. Tiếp theo, công thức môi trường ½ MS bổ sung 0,5 mg/l NAA thích hợp cho tạo rễ in vitro với tỷ lệ hình thành rễ cao nhất đạt 67,50%. Cây in vitro được trồng trên giá thể hỗn hợp TS 1 cho tỷ lệ sống cao (75,5%) sau 2 tuần rèn luyện., Tóm tắt tiếng anh, Rose is considered as one of the most important cut flower species in the world and in Vietnam. In this study, stem segments of rose obtained from Me Linh (Ha Noi) were used as the materials for the micropropagation. The results showed that all of the treatments supplemented BAP were stimulated generating shoot and the concentration of 1.0 mg/L BAP was found as the best formula for the growth of regenerated shoots. The formula of MS with 1.0 mg/L BAP was highly recommended for the multiplication of rose nodal explants as the shoots number per explant were 3.80, the length of shoot was 1.70 cm and the leaves number per shoot reached 4.20. In the case of adding 0.2 mg/L activated charcoal, the length of newly regenerated shoots were found to be the highest one (approximately 2.70 cm) as compared with other formulas. Next, the medium ½ MSwith 0.50 mg/L NAA showed the efficiency for the in vitro root formation, with 67.50 roots per microshoot. Finally, in vitro plantlets were acclimatized by growing in TS 1 mixture substrate which exhibited the highest survival rate by approximately 75.5% after 2 weeks.

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