EFFECTIVE RECOVERY AND PURIFICATION OF POLY(3-HYDROXYBUTYRATE) FROM A HALOPHILIC BACTERIUM BY CHEMICAL DIGESTION METHOD

• Tác giả: Van Thuoc Doan, Thi Loan Tran
• Ngôn ngữ: Vie
• Thông tin xuất bản: Tạp chí Khoa học Trường Đại học Sư phạm Hà Nội: Khoa học Tự nhiên, 2019
• Bộ sưu tập: Metadata
• ID: 408354

Tóm tắt

A simple and effective process for the recovery of intracellularpoly(3-hydroxybutyrate) (PHB) from a halophilic bacterial strain - Salinivibrio sp.M318 was developed using a chemical digestion method. The effect oftemperature, chemicals (sodium hypochlorite and sodium hydroxide) and theirconcentration on PHB recovery was examined. It was found that sodium hydroxidewas an effective chemical for the recovery of PHB from Salinivibrio sp. M318.High PHB recovery yield of 97% and polymer purity of 99% were obtained when50 g/L of bacterial cells were incubated in NaOH solution at the concentration of0.075 M for 1 h at 50oC. It is expected that this simple method can be of interestfor other PHA production processes.