Tạo tấm tế bào sừng từ tế bào máu cuống rốn trên giá thể màng ối

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Tác giả: Điền Hồ, Thị Mỹ Linh Lương, Công Toại Trần, Thị Thanh Thủy Trần

Ngôn ngữ: Vie

Ký hiệu phân loại: 660.65 Genetic engineering

Thông tin xuất bản: Y Dược học Cần Thơ 2023

Mô tả vật lý: 407-413

Bộ sưu tập: Metadata

ID: 408398

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Tạo tấm tế bào sừng nhiều lớp trên giá thể màng ối. Đối tượng và phương pháp nghiên cứu: Nghiên cứu thực nghiệm mô tả. Đối tượng nghiên cứu là máu cuống rốn và màng ối từ bánh nhau của trẻ mới sinh. Thu nhận tế bào gốc trung mô từ máu cuống rốn, định danh, nuôi cấy tăng sinh tế bào. Thu nhận, xử lý màng ối thành giá thể mang tế bào. Chuyển tế bào gốc trung mô lên màng ối và tiến hành biệt hóa thành tế bào sừng khi mật độ tế bào đạt 60-80% diện tích. Quan sát dưới kính hiển vi đảo ngược ghi nhận sự thay đổi hình dạng tế bào. Sự biến đổi hình dạng tế bào hay sự biệt hóa đạt 80% tiến hành quá trình tạo tầng bằng phương pháp airlifting. Quá trình được thực hiện trong 7 ngày. Sản phẩm được đánh giá: hình thái, sự biểu hiện các marker P63, CK 5/6 và sự liên kết tế bào với tế bào, tế bào với giá thể qua phương pháp chụp TEM. Kết quả: Thu nhận đúng tế bào gốc trung mô từ máu cuống rốn. Thu nhận, xử lý thành công màng ối thành giá thể mang tế bào. Tạo được tấm tế bào sừng có 3-5 lớp tế bào. Kết luận: Tạo thành công tấm tế bào sừng có nhiều lớp tế bào trên giá thể màng ối., Tóm tắt tiếng anh, Products of biological origin effective response in the treatment of burns and skin lesions loss is a matter of urgency. we proceed the study:"Create keratinocytes plate from cord blood stem cells on the substrate amnion". Objectives: Create keratinocytes plate with multiple layers on the substrate amnion. Materials and method: The study was designed experimental description. Subjects of study were cord blood and amniotic membranes from the placenta of a fullterm newborn. We collected mesenchymal stem cells from umbilical cord blood, identify and cultivate cell proliferation. Receiving and processing of the amniotic membrane into a carrier cell. Mesenchymal stem cell transplantation from umbilical cord blood to amnion. When the cell density reaches 60-80% of the area, we performed differentiation of mesenchymal stem cells into skeratinocytes. Observe under the reverse microscope to record the change in cell shape. Cell shape change reached 80%. We conducted the cascading process by airlifting method. The airliffting process was done in 7 days. Products evaluated: morphology (HE), expression of P63, CK 5/6, and TEM. Result: Successfully obtained mesenchymal stem cells from umbilical cord blood. Receive and treat the membranes successfully into cell-bearing media. Successfully create keratinocytes plate with 3-5 layers of cells. Conclusion: Successful generation of keratinocytes plate with multiple layers on the substrate amnion.

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