Nhân nhanh chồi và tạo cây chuối già Nam Mỹ (musa acuminata cavendish) hoàn chỉnh bằng kỹ thuật nuôi cấy in vitro

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Tác giả: Hải Châu Mai

Ngôn ngữ: Vie

Ký hiệu phân loại: 576.5 Genetics

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ Lâm nghiệp, 2023

Mô tả vật lý: 45649

Bộ sưu tập: Metadata

ID: 408477

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Tóm tắt
Chủ đề

Nghiên cứu này tiến hành khảo sát nồng độ các chất điều hòa sinh trưởng thực vật phù hợp cho nhân giống in vitro cây chuối già Nam Mỹ, tác động của giá thể và phân bón đến quá trình ươm trồng cây con in vitro bên ngoài vườn ươm. Chồi cây chuối nhân nhanh tốt nhất trên môi trường MS bổ sung BA 4 mg/L, đường 30 g/L, agar 6 g/L. Sau 30 ngày nuôi cấy, tỷ lệ mẫu tạo chồi đạt 100%, số chồi thu được là 5,3 chồi/mẫu, chiều cao 2,08 cm. Chồi thu được ở giai đoạn nhân nhanh sau đó được chuyển sang giai đoạn tạo rễ cho thấy môi trường bổ sung hay không bổ sung IBA đều cho khả năng ra rễ. Tuy nhiên môi trường MS bổ sung IBA 1,5 mg/L, đường 30 g/L, 6 g/L agar cho kết quả tạo rễ tốt nhất (7,73 rễ), chiều dài dễ và chiều cao cây lần lượt là 5,67 cm và 7,47 cm. Cây chuối già Nam Mỹ in vitro sau đó được đưa ra trồng bên ngoài vườn ươm và sinh trưởng tốt nhất trên giá thể đất : mụn dừa (tỷ lệ 1:1), tỷ lệ sống đạt 93,3%, cây cao 16,5 cm và có số lá trung bình là 5,4 lá. Bón phân NPK (20-15-5) nồng độ 5g/L, 7 ngày/lần sau khi trồng cây ra vườn ươm giúp cây sinh trưởng tốt, tỷ lệ cây sống là 93,3%, cây đạt chiều cao 16,2 cm và 5,2 lá., Tóm tắt tiếng anh, With an aim at the propagation of the Musa acuminata Cavendish, the effects of the plant growth regulation on the shooting and the rooting stage, growing media and fertilizer concentrations in the greenhouse stage were investigated. The in vitro single shoots were planted on the MS medium supplemented with BA 4 mg/l, sucrose 30 g/l, agar 6g/L. After 30 days of culture, the highest number of shoots was 5.3 shoots/explants and the shoots' average height was 2.08 cm. Afterwards, the in vitro shoots were inoculated for the rooting stage on the MS medium which was supplemented or not with IBA. The results of root production showed that MS medium supplemented with IBA 1:5 mg/l, sucrose 30 g/l, 6g/L agar created the highest number of roots (7.73 roots), root length and plant's height was 5.67 cm và 7.47 cm respectively. The in vitro plants were planted in the greenhouse with different combinations of growing media and fertilizer concentrations. The mixture of soil and coconut fiber with the ratio 1:1 were acquired in 93.3% of in vitro plants successfully planted and growing, the plants' height and average leaf were 16.5 cm and 5.4 leaves, respectively. 5 g/l of NPK (20-15-5) lead to the best growth of the Musa acuminata Cavendish, which were 93,3% successfully planting
16.2 cm height and 5.2 leaves/explant.

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