Nghiên cứu nuôi cấy in vitro cây cẩm cù tên lửa (Hoya multiflora)

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Tác giả: Thị Thu Ngà Nguyễn, SYHALAHT Soneseo, Thị Hồng Trần

Ngôn ngữ: Vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ Đại học Thái Nguyên, 2021

Mô tả vật lý: 154-162

Bộ sưu tập: Metadata

ID: 409268

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Lan Cẩm cù tên lửa (Hoya multiflora) là cây cảnh trang trí rất đẹp. Ngoài ra cây còn có tác dụng rất tốt trong chữa một số bệnh, đã được khoa học các nước chứng minh. Việc bảo tồn các giống cây dược liệu nói chung và cây Cẩm cù tên lửa nói riêng có thể thực hiện bằng nhiều biện pháp truyền thống như gieo hạt sau khi thu hái trái chín già, có thể chọn lá hoặc thân già đã có rễ để giâm xuống đất. Tuy nhiên hiệu quả đạt được chưa cao do ít khi cây tạo quả chín để lấy hạt. Trong nghiên cứu này, chúng tôi sử dụng phương pháp nuôi cấy in vitro nhằm tìm ra môi trường nuôi cấy thích hợp cho sự sinh trưởng và phát triển của cây trong ống nghiệm. Kết quả thu được, môi trường có bổ sung BAP 1,0 mg/l cho tỷ lệ tạo đa chồi tốt nhất trong các nồng độ thử nghiệm. Trên môi trường này, chồi xanh đậm, phát triển tốt (3,28 chồi/mẫu). Môi trường tối ưu cho quá trình kéo dài chồi là MS bổ sung GA3 0,5 mg/l (4,84 cm/mẫu). Môi trường thích hợp nhất cho tạo cây hoàn chỉnh là MS bổ sung α-NAA với nồng độ 0,5 mg/l. Số rễ thu được đạt 4,83 rễ/chồi sau 8 tuần. Tỷ lệ cây con sống sót khi trồng trên giá thể ngoài tự nhiên đạt 88,3%., Tóm tắt tiếng anh, Hoya multiflora is a beautiful ornamental plant decoration. In addition, Hoya multiflora also works very well in treating a number of diseases, which has been scientifically proven. The conservation of medicinal plants in general and Hoya multiflora in particular can be done by many traditional methods such as sowing seeds after harvesting old ripe fruits and choosing leaves or old stalks with roots to planted to the ground. However, the achieved effect is not high, rarely has ripe fruit to get seeds. In this study, we used in vitro culture method to find a culture medium suitable for plant growth and development in vitro. The results showed that the medium supplemented with BAP 1.0 mg/l gave the best multi-shoot efficiency in the in vitro concentrations of Hoya multiflora. In this medium, green shoots, well developed (3.28 shoots/sample). Optimal medium for the elongation of shoots supplemented with GA3 0.5 mg/l (4.84 cm/sample). The complete plant formation is most suitable on basal MS medium supplemented with α-NAA at a concentration of 0.5 mg/l. The number of roots obtained was 4.83 roots/sample after 8 weeks. The survival rate of seedlings when planted on natural substrates reached 88.3%.

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