Tái sinh chồi cây dưa lưới (Cucumis melo L.) in vitro từ tử diệp

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Tác giả: Thị Như Cao, Minh Lý Lê, Văn Ây Nguyễn, Thị Bích Thủy Võ

Ngôn ngữ: Vie

Ký hiệu phân loại:

Thông tin xuất bản: Khoa học (Đại học Cần Thơ) 2023

Mô tả vật lý: 114-122

Bộ sưu tập: Metadata

ID: 409748

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Tóm tắt
Chủ đề

Nghiên cứu được thực hiện nhằm tìm ra nồng độ các chất điều hòa sinh trưởng (BA, Kinetin, IBA và NAA) thích hợp cho quá trình tái sinh chồi, nhân chồi và tạo rễ in vitro của chồi dưa lưới tái sinh từ tử diệp. Thí nghiệm được thực hiện tại Phòng nuôi cấy mô của trường Nông nghiệp, trường Đại học Cần Thơ. Nghiên cứu gồm 3 thí nghiệm được bố trí theo thể thức hoàn toàn ngẫu nhiên 2 nhân tố gồm tái sinh chồi trực tiếp từ tử diệp, nhân chồi và tạo rễ in vitro cây dưa lưới. Kết quả cho thấy tử diệp ở vùng gần phôi (3 ngày sau khi gieo) nuôi cấy trên môi trường MS bổ sung BA 0,5 mg/L cho tỷ lệ tái sinh chồi là 97,5% sau 3 tuần nuôi cấy
môi trường MS + Kinetin 1,0 mg/L + BA 0,5 mg/L cho hiệu quả nhân chồi dưa lưới tốt nhất với 3,4 chồi
môi trường MS bổ sung IBA 0,5 mg/L thích hợp tạo rễ in vitro cây dưa lưới tái sinh từ tử diệp (78,1% và 3,8 rễ) sau 3 tuần., Tóm tắt tiếng anh, This study was carried out to determine the optimum concentrations of plant growth regulators (BA, kinetin, IBA and NAA) which are suitable for shoot regeneration from cotyledons, multiplication and rooting of cantaloupe in vitro. The experiments were taken place in Laboratory of Plant Tissue Culture, College of Agriculture, Can Tho University. It consisted of three experiments using completely randomized design with two factors including shoot regeneration from cotyledons sections, shoot proliferation, and rooting of cantaloupe in vitro. The results showed that the cotyledons at proximal (seedlings 3 days after sowing) on Murashige and Skoog medium supplemented with BA 0.5 mg/L obtained a high rate of shoot regeneration 97.5% after 3 weeks
Murashige and Skoog medium supplemented with Kinetin 1.0 mg/L and BA 0.5 mg/L was effective for the rapid proliferation of shoots in vitro with 3.4 shoots and IBA 0.5 mg/L was favorable for root induction (78.1% and 3.8 roots) of cantaloupe shoots which were regenerated in vitro from cotyledons.

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