Phân tích in-silico xác định và mô tả họ gen kháng NBS-LRR ở chuối Musa acuminata

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Hồng Lĩnh Tạ, Đức Trung Trần

Ngôn ngữ: Vie

Ký hiệu phân loại: 635 Garden crops (Horticulture) Vegetables

Thông tin xuất bản: Khoa học và công nghệ Việt Nam , 2021

Mô tả vật lý: 52 - 58

Bộ sưu tập: Metadata

ID: 410017

Thêm vào giỏ Liên kết toàn văn

Bệnh hại do vi khuẩn, virus là mối đe dọa nghiêm trọng đối với ngành sản xuất chuối trên thế giới nói chung, Việt Nam nói riêng. Ngoài các biện pháp canh tác, việc khai thác và ứng dụng nguồn gen kháng - phần lớn thuộc họ gen NBS-LRR (Nucleotide binding site - Leucine rich repeat) trong chọn tạo, cải thiện giống chuối là phương thức bền vững để đối phó với dịch bệnh. Hệ gen loài chuối Musa acuminata được giải mã tạo tiền đề cho các nghiên cứu di truyền chuyên sâu, bao gồm xác định họ gen kháng NBS-LRR. Trong nghiên cứu này, các công cụ tin sinh học đã được sử dụng để xác định, mô tả cấu trúc và quá trình tiến hóa của 97 gen NBS-LRR. Ngoại trừ gen Ma_NBS_083 thuộc phân họ RNL, các gen kháng còn lại ở chuối M. acuminata thuộc phân họ CNL và hầu hết là gen chứa một exon. 66 gen NBS-LRR (68%) được định vị trên 11 nhiễm sắc thể (NST), 38 gen trong số đó (39%) hình thành các cụm gen. Các gen NBS-LRR của chuối được chia thành 7 phân nhóm dựa trên sự đa dạng về trình tự và số lượng, cấu trúc các motif bảo thủ đặc trưng cho các vùng chức năng. Kết quả này cung cấp cơ sở khoa học cho các nghiên cứu đánh giá biểu hiện gen kháng và khai thác ứng dụng trong chọn tạo giống chuối. , Tóm tắt tiếng anh, Various common diseases caused by bacteria and viruses are increasing threats to the production of bananas worldwide. Besides the practical application of cultivation techniques, intensive exploitation of resistant genes, most of which belong to the NBS-LRR (Nucleotide-binding site - Leucine-rich repeat) gene family, in fortifying banana's disease resistance, has been considered as a sustainable approach. The recently published genome assembly of Musa acuminata, a species of the Musa genus native to Southern Asia, has facilitated the genome-wide identification and characterisation of NBS-LRR genes. In the present study, an in-silico approach was employed to identify and characterise 97 NBS-LRR genes and their evolution in M. acuminata genome. Except Ma_NBS_083 belonging to the RNL sub-family, other identified genes are members of the sub-family CNL and most of them contain only one exon. Sixty-six (66) NBS-LRR genes (68%) were mapped on 11 banana chromosomes, among them 38 genes (39%) located in clusters. Phylogenetic analysis in combination with conserved motif identification classified the banana NBS-LRR genes into seven groups. This study provides novel insight into the NBS-LRR gene family in bananas as a potential resource for further research in functional evaluation and utilisation of resistant genes.

1. Đọc trực tuyến

1. Đọc trực tuyến

Tạo bộ sưu tập với mã QR