Hiệu quả ức chế virus gây bệnh Gumboro của interferon trên gà thực nghiệm

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Tác giả: Quảng Đồ Hồ, Đăng Quân Nguyễn, Thị Thanh Giang Nguyễn

Ngôn ngữ: Vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí khoa học & công nghệ Việt Nam 2020

Mô tả vật lý: 48 - 53

Bộ sưu tập: Metadata

ID: 412039

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Nghiên cứu hiệu quả điều trị bệnh Gumboro của interferon gà tái tổ hợp (recombinant chicken interferon, rChIFN) khi sử dụng chỉ mỗi interferon alpha gà (rChIFN-α) hay có sự kết hợp với interferon gamma (ChIFN-γ) được thực hiện trên gà 3 tuần tuoi. Đầu tiên gà đưoc công cường độc với virus Gumboro độc lực cao (1x10 ELD50 môi con) bằng cách nhỏ mắt, nhỏ mũi sau 8 giờ xử lý với virus, gà được điều trị bằng cách nhỏ mắt và nhỏ mũi với rChFN ứng với 1 trong 6 nhóm nghiệm thức. Nhóm sử dụng rChIFN-α 100 μg/con, tỷ lệ gà đưoc bão hộ là 56,67%, tỷ lệ sông là 93,33% nhóm sử dụng rChIFN-a 100 μg/con kết hợp rChIFN-y (1 g/con) tỷ lệ gà được bảo hộ là 70,00%, tỷ lệ sông là 93,33% nhóm sử dụng rChIFN-α 10 μg/con tỷ lệ gà được bảo hộ là 36,67%, tỷ lệ sống là 80,00% nhóm sử dụng ChIP-a 10 ug/con kết hợp ChIFN-γ (1 µg/con) ty lệ gà được bảo hộ là 53,33%, tỷ lệ sống là 86,67%. Trong khi đó, nhóm đối chứng dương (gà nhiễm virus, không đưoc điều trị), gà không được bảo hộ (tỷ lệ nhiễm bệnh là 100%) và tỷ lệ sống chỉ đạt 60,00% đối chứng âm (gà không nhiễm virus, không xử lý với rChIFN) gà hoàn toàn không nhiễm bệnh và tỷ lệ sống 100%. Kết quả này cho thấy, sử dụng rChIFN-α làm tăng tỷ lệ bảo hộ, tỷ lệ sông khi gà bị nhiễm bệnh Gumboro theo nồng độ sử dụng. Đồng thời sử dụng rChIFN-α kết hợp rChlFN-γ đã làm tăng hiệu quả điều trị so với sử dụng chỉ mỗi rChIFN-α, rChIFN- γ, Tóm tắt tiếng anh, A study on the efficiency of recombinant chicken interferon (rChIFN) in the treatment of Gumboro disease by using only chicken interferon alpha (ChIFN-α) or combining with interferon gamma (ChIFN-γ) was carried out in 3-week-old chickens. The experiment was performed as the following procedure: firstly, chickens were exposed to eye/nasal drops with virulent Gumboro disease virus with a dose of 1x105 ELD50 per chick. After 8 hours of challenges virus, chickens were exposed to eye/nasal drops with rChIFN-α for one of the six experimental groups. The chickens were used rChIFN-α 100 μg/chick having the protection and the survival rate in respectively 56.67% and 93.33%. The chickens were used rChIFN-α 10 μg/chick combining with rChIFN-γ (1 μg/chick) having the protection and the survival rate in respectively 70.00% and 93.33%. The chickens were used rChIFN-α 10 μg/chick having the protection and the survival rate in respectively 36.67% and 80.00%. The chickens were used rChIFN-α 10 μg/chick combining with rChIFN-γ (1 μg/chick) having the protection and the survival rate in respectively 53.33% and 86.67%. Meanwhile, the positive control group (chickens were infected, untreated), chickens were not protected (100% infection rate) and survival rate was only 60.00% negative control group (non-viral chickens, not treated with rChIFN), whole chickens were not infected and 100% survival rate. The results suggested that using rChIFN-α increased the protection and survival rate of Gumboro infected chickens depending on rChIFN concentration. Concurrently, using rChIFN-α combined with rChIFN-γ had increased the treatment efficiency compared to using only rChIFN-α.

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