Antioxidant activity is an importance factor for assessing the applicability of plant extracts in the food,functional foods and cosmetics sectors. Codonopsis pilosula Franch. is a tranditional herb wasextracted and freezing dry for storage, evaluation of their antioxidant activity Ampelopsis cantoniensisK.Koch, and Piper betle L. and comapre with extracts of Ampelopsis cantoniensis K.Koch, and Piperbetle L. The antioxidant activity of these herbs were evaluated by total phenolic, total flavonoid, DPPHand ABTS•+radical scavenging methods. Results showed that the total phenolics and flavonoids ofCodonopsis pilosula Franch are smallest among three herbs. The DPPH radical scavenging activity ofAmpelopsis cantoniensis is better than that of other herbs, and has the lowest EC50 values. Ampelopsiscantoniensis K.Koch, and Piper betle L., also showed a high ABTS•+radical cation scavenging activityand the activity of three herb extracts are in the same order as DPPH• radical scavenging activity.Expecially, Ampelopsis cantoniensis and Piper betle have a higher radical scvenging activity thanascorbic acid. Arcoding to the eastern medicine, Codonopsis pilosula Franch. is a medical forimproving health, supporting digestive system, and circulatory system, however, the extract of thisherb did not show noticeable antioxidant activities, it is recomnened to combind with a high antioxidantactivity extract from other herbs for stablizing and protecting bioactive compounds.Keywords natural antioxidant, Ampelopsis cantoniensis K.Koch, Piper betle L., and Codonopsispilosula Franch.), phenolic, flavonoid, radical scavenging