Nghiên cứu xây dựng quy trình nhân giống in vitro loài lan brassavola nodosa

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Tác giả: Quỳnh Anh Phạm, Thị Thu Nhi Phạm

Ngôn ngữ: Vie

Ký hiệu phân loại: 576.5 Genetics

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ Đại học Thái Nguyên, 2022

Mô tả vật lý: 277-284

Bộ sưu tập: Metadata

ID: 412979

Thêm vào giỏ

Brassavola nodosa thuộc họ Phong lan (Orchidaceae) có hoa rất đa dạng về màu sắc, hình dáng, đặc biệt về hương thơm. Trong nghiên cứu này, chồi non lan Brassavola nodosa có chiều cao từ 7 - 10 cm được khử trùng bề mặt. Sau đó, chồi có kích thước 2 cm không bị nhiễm sẽ tách ra tái sinh và nhân nhanh. Kết quả cho thấy, môi trường MS bổ sung BA 1,5 mg/L và NAA 0,25 mg.L-1 cho tỉ lệ mẫu tạo chồi là 97,78 %, số chồi phát sinh là 16,89 chồi/mẫu. Tần suất ngập chìm và thời gian ngập chìm thích hợp cho sự nhân nhanh chồi trong hệ thống nuôi cấy ngập chìm tạm thời RITA® là 6 lần/ngày và thời gian ngập là 1 phút cho chỉ số tăng trưởng chồi đạt 14,48 lần. Môi trường khoáng Knudson C (1946) phù hợp cho sự ra rễ của chồi in vitro. Tỉ lệ sống sót của cây ở giai đoạn vườn ươm sau 4 tuần trồng đạt 100%., Tóm tắt tiếng anh, Brassavola nodosa belongs to the Orchidaceae with flowers that are various in color, shape, and especially fragrance. Brassavola nodosa are being interested in developing planting areas, creating valuable genetic resources for hybridization, and selecting and creating new orchid varieties. In this study, 7-10 cm young shoots of Brassavola nodosa were surface sterilized and then taken 2 cm disinfected apex for rapid regeneration and multiplication. The results showed that MS media supplemented BA 1.5 mg.L-1 and NAA 0.25 mg.L-1 was suitable for in vitro shoot regeneration, the rate of shoot regeneration reached 97.78%, the number of generated shoots per explants was 16.89. The frequency of submergence and the duration of submergence for rapid shoot multiplication in the RITA® temporary submerged culture system were 6 times/day and 1 minute, repectively. The multiplication coefficient of shoot reached 14.48 times. Knudson C Mineral Medium (1946) was suitable for rooting of in vitro shoots. The survival rate of plants in the nursery period after 4 weeks of planting was 100%.

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