RESEARCH ON PROCEDURE THE MICROPROPAGATION FOR ORCHID SPECIES KIEM "PHAN TRI" (Cymbidium finlaysonianum Lindl.)

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Tác giả: Thị Kim Huỳnh, Thị Điệp Nguyễn

Ngôn ngữ: Vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ Đại học Thái Nguyên, 2020

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ID: 413723

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Lan Kiếm Phan Trí (thuộc họ Cymbidium) là loài lan Kiếm Tiên Vũ hoa bán sơn địa lá dài, rất cứng, hoa vàng đột biến rất nổi tiếng của Việt Nam. Trong nghiên cứu nhân giống cây lan Kiếm Phan Trí này, chồi non có chiều dài từ 20 - 30 cm được sử dụng làm vật liệu nuôi cấy khởi đầu. Khi chồi in vitro đạt kích thước 2 cm, tách lấy chồi đỉnh để làm vật liệu cho các thí nghiệm. Kết quả cho thấy, trên môi trường MS có bổ sung BA 3 mg/l và NAA 0,5 mg/l cho tỷ lệ mẫu tạo PLBs là 64,44% và số lượng PLBs là 17,2 PLBs/mẫu. Kiểu nuôi cấy bằng hệ thống nuôi cấy ngập chìm tạm thời RITA® với sự gia tăng trọng lượng PLBs và số chồi tái sinh tương ứng là 18,7 lần và 84,6 chồi. Môi trường khoáng Hyponex® với thành phần NPK là 202020 kết hợp với 15 g/l chuối sứ chín thu được 100% chồi ra rễ, chiều cao cây là 6,3 cm và có 3,67 rễ. Tỷ lệ sống của cây giai đoạn vườn ươm sau 1 tháng trồng đạt 95%., Tóm tắt tiếng anh, The orchid species Kiem Phan Tri (belonging to genus Cymbidium) is a species of orchid that grows very hard and long leaves, mutant yellow flowers that have been famous for a long time in Vietnam. In the study of propagating the orchid species Kiem Phan Tri, young shoots with length of 20 - 30 cm were used to the starting culture material. When dormant buds reached 2 cm, their tops were cut and used as experiment materials. The results showed that on MS medium containing 3 mg/l BA and 0.5 mg/l NAA, the precentage of PLBs regeneration from the top of buds was 64.44% and 17.2 PLBs/explant. The type of culture using the RITA® temporary was submerged culture system, with the increase in the PLBs weight and the number of regenerated shoots was 18.7 times and 84.6 shoots respectively. Hyponex® medium with NPK consist of 202020 combining with 15 g/L bananas for a rooting rate of 100% showed the shoots height are 6.3 cm with 3.67 roots. Survival rate of plantlets in nursery garden was 95%.

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