STUDY PROPAGATATION OF TECHTONA GRANDIS LINN (STRAINS VN34 and VN19) BY TISSUE CULTURE METHOD

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Tác giả: Thị Hồng Nguyễn, Thị Phương Vũ

Ngôn ngữ: Vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ Đại học Thái Nguyên, 2019

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Bộ sưu tập: Metadata

ID: 417190

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Nghiên cứu nhân giống cây Tếch dòng VN34 và VN19 bằng phương pháp nuôi cấy mô tế bào thực vật, bao gồm nghiên cứu Nồng độ thích hợp chất khử trùng chồi nách, nghiên cứu chất kích thích sinh trưởng đến hệ số nhân chồi và tỷ lệ nảy chồi, chất kích thích ra rễ chồi Tếch. Môi trường thí nghiệm nhân chồi nách cây Tếch dòng VN34 và VN19 là MS*. Kết quả, trên nền môi trường MS*, sử dụng chất khử trùng 0,1% HgCl2 10 phút, thu được chất lượng chồi tốt nhất với các chỉ tiêu nghiên cứu (hệ số nhân chồi - HSNC, chiều cao chồi - CCC, tỷ lệ ra rễ - TLRR, chiều dài rễ - CDR, số rễ trung bình - SDTB). Chất kích thích sinh trưởng được bổ sung là tổ hợp giữa BAP, NAA (0,75 mg/l BAP + 0,25 mg/l NAA) cho kết quả nhân nhanh chồi tốt nhất so với sử dụng riêng BAP hay Kinetin. Chất kích thích ra rễ là IBA cho tỷ lệ ra rễ hiệu quả nhất với nồng độ 0,75 mg/l IBA., Tóm tắt tiếng anh, Study on the propagation of Tectona grandis L. f. (Teak) two strains VN34 and VN19 by the method of cultivating plant cell tissue, including the study appropriate concentration of axillary bud disinfectant, growth stimulants to shoot multiplier, stimulation of Teak roots. The medium was used for the axillary buds of the teak strains VN34 and VN19 as MS*. As a result, on MS* medium, using disinfectant 0.1% HgCl2 for 10 minutes, obtained the best axillary bud quality (shoot multiplier - SM, shoot height - SH, ratio rooting- RR, root length - RL, average roots - AR). The growth stimulant added was a combination of BAP, NAA (0.75 mg/l BAP+0.25 mg/l NAA), which resulted in the best shoot multiplication compared to using BAP or Kinetin alone. The root stimulant is IBA was the most effective rooting rate with a concentration of 0.75 mg/l IBA.

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