GIẢI TRÌNH TỰ GEN rpoB VÀ NHÂN NHANH CÂY OẢI HƯƠNG LÁ XẺ BẰNG KỸ THUẬT NUÔI CẤY MÔ

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Tác giả: Đức Hà Chu, Việt Hồng La

Ngôn ngữ: Vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ Đại học Thái Nguyên, 2020

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ID: 417952

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Tóm tắt
Chủ đề

Trong nghiên cứu này, gen rpoB phân lập từ loài oải hương lá xẻ (Lavanduladentata) thu thập tại Đà Lạt (Lâm Đồng) đã được giải trình tự làm cơ sở để xây dựng cây phả hệ. Nhân giống cây oải hương bằng kỹ thuật nuôi cấy mô từ đốt thân cũng được hoàn thiện. Kết quả cho thấy, gen rpoB của L. dentata có kích thước 483 bp. Phân tích sơ đồ hình cây cho thấy loài oải hương lá xẻ trong nghiên cứu này xếp cùng nhóm với loài L. angustifolia. Đốt thân của oải hương lá xẻ được khử trùng bề mặt bằng dung dịch javel 5% trong 10 phút. Môi trường thích hợp để tái sinh và nhân nhanh chồi in vitro là MS, agar 7 g.L-1, sucrose 30 g.L-1 bổ sung BAP 0,7 mg.L-1. Số chồi/mẫu đạt 10,13
số lá/chồi đạt 9,66 và chiều cao chồi đạt 3,28 cm sau 8 tuần nuôi cấy. Môi trường phù hợp để ra rễ in vitro là MS, agar 7 g.L-1, sucrose 30 g.L-1 bổ sung NAA 0,5 mg.L-1, cho số rễ trung bình/chồi là 14,66, chiều dài rễ 1,58 cm sau 2 tuần nuôi cấy. Giá thể đất + xơ dừa (11) thích hợp để rèn luyện cây oải hương cấy mô, cho tỷ lệ sống đạt 65,0% sau 3 tuần rèn luyện., Tóm tắt tiếng anh, In this study, the rpoB gene of L. dentata which was obtained from Da Lat (Lam Dong), was sequencing. It was used to construct the phylogenetic tree. The propagation of L. dentata by tissue culture technique from stem segments was improved. Results showed that the rpoB gene of L. dentata was sequenced, this gene was 483 bp in the length. The analysis of phylogenetic tree expressed that lavender in this work was in the group with L. angustifolia. Stem segments of L. dentata were surface sterilized by 5% javel solution in 10 minutes. The suitable medium for shoots regeneration and multiplication was MS 7 g.L-1 agar, 30 g.L-1 sucrose added 0.7 mg.L-1 BAP. The shoot number per explants was 10.13, the leaf number per shoot was 9.66 and the shoot length was 3.28 cm after 8 weeks of culture. The favorable medium for in vitro rooting was MS, 7 g.L-1 agar, 30 g.L-1 sucrose supplemented 0.5 mg.L-1 NAA. The root number per shoot was 14.66 and the length of root was 1.58 cm after 2 weeks culture. The mixture of soil and coconut fiber (1 1) was suitable for hardening of in vitro lavender, the survival rate reached 65.0% after 3 weeks of acclimatization.

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