Ảnh hưởng môi trường nuôi cấy đến sinh trưởng hệ sợi của chủng nấm Phellinus PHE67

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Tác giả: Thanh Uyên Lê, Duy Trình Nguyễn, Văn Giang Nguyễn, Thị Huệ Tạ, Đông Anh Trần, Thu Hà Trần

Ngôn ngữ: Vie

Ký hiệu phân loại: 633 Field and plantation crops

Thông tin xuất bản: Nông nghiệp và Phát triển nông thôn, 2020

Mô tả vật lý: 52 - 58

Bộ sưu tập: Metadata

ID: 417979

Thêm vào giỏ Liên kết toàn văn

Chi nấm Phellinus spp. là một chi nấm có giá trị dược liệu cao được sử dụng nhiều trong các nghiên cứu y khoa. Ở Việt Nam, nghiên cứu về nấm Phellinus mới chỉ dừng lại ở phân lập, tuyển chọn. Các nghiên cứu cơ bản về tối ưu hóa các điều kiện nuôi cấy còn rất sơ khai. Nghiên cứu này được tiến hành nhằm đánh giá ảnh hưởng của yếu tố dinh dưỡng đến sinh trưởng hệ sợi của chủng nấm Phellinus PHE67 được thu thập tại Hà Nội. Môi trường nuôi cấy thuần khiết thích hợp cho hệ sợi nấm PHE67 là PGA (200 g/l khoai tây, 20 g/l glucose, 15 g/l agar). Trong số các nguồn dinh dưỡng cacbon nghiên cứu, fructose là phù hợp nhất cho sự sinh trưởng hệ sợi nấm PHE67, tốc độ phát triển hệ sợi đạt 2,96 mm/ngày. Sinh trưởng của Phellinus PHE67 kém nhất ở nguồn dinh dưỡng lactose. Dinh dưỡng NH4NO3 cho hiệu quả kích thích tăng trưởng hệ sợi nấm PHE67 cao nhất với tốc độ 3,52 mm/ngày, hệ sợi nấm dày. MgSO4 cũng được xác định là nguồn dinh dưỡng khoáng tối ưu nhất cho chủng PHE67., Tóm tắt tiếng anh, Phellinus spp. is the genus which has a great medicinal value and was applied in massive medicinal research. In Vietnam, researches on Phellinus have just been only in the step of isolation and selection of new strains from nature, while the fundamental researches in optimizing the cultural conditions, time of incubation, and effects of the mineral on mycelial growth have still lacked. This study aims to investigate the influence of nutritional factors on mycelium growth of mushroom Phellinus PHE67 which was collected in Bach Thao park of Hanoi. The results show that the suitable medium for growth of PHE67's mycelia is PGA containing extracts of 200 g/l of potatoes, 20 g/l glucose and 15 g/l of agar. Among the carbon sources, fructose was seemed to be the most appropriate for growing of PHE67's mycelia, the growth was recorded at 2.96 mm/day while the slowest growth of PHE67's mycelia was observed in the case of lactose source. NH4NO3 is demonstrated to stimulate the highest growth of PHE67, at 3.52 mm/day. On this media the mycelia are thick, compact and evenly growing in all directions. Furthermore, the most optimal mineral source for PHE67 is MgSO4.

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