Survey on microbial contamination, characteristics of E. coli bacteria in pork samples collected in Dien Bien province

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Tác giả: Văn Tuyên Nguyễn

Ngôn ngữ: Vie

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Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2019

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Bộ sưu tập: Metadata

ID: 418241

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Chúng tôi đã phân tích 90 mẫu thịt lợn thu tại các chợ bán lẻ của tỉnh Điện Biên từ tháng 6/2016 - 4/2017 để khảo sát mức độ ô nhiễm về chỉ tiêu tổng số vi khuẩn hiếu khí (TSVKHK) và E. coli ở thịt lợn. Kết quả phân tích cho thấy có 98,89% mẫu thịt dương tính với TSVKHK, trong đó tỷ lệ mẫu không đạt tiêu chuẩn vệ sinh là 48,89%. Có 75,56% mẫu thịt nhiễm E. coli với tỷ lệ mẫu không đạt tiêu chuẩn an toàn thực phẩm về mức nhiễm E. coli dao động từ 30% - 53,33% và số lượng vi khuẩn E. coli nhiễm trong các mẫu thịt này dao động trong khoảng 1,03 x 102 CFU/g đến 5,23 x 102 CFU/g. Trong đó, thịt lợn bán ở chợ trung tâm có tỷ lệ và cường độ nhiễm khuẩn vượt quá giới hạn cho phép là cao nhất. Tỷ lệ nhiễm khuẩn E. coli tăng dần và tỷ lệ thuận với thời gian sau giết mổ và tăng trong mùa Hè - Thu so với mùa Đông - Xuân. Các chủng vi khuẩn E. coli phân lập được mang đầy đủ các đặc tính sinh vật, hóa học đặc trưng của giống và được xác định là có khả năng gây độc cho con người. Kết quả kiểm tra mức độ mẫn cảm với kháng sinh của 68 chủng vi khuẩn phân lập cho thấy có 67,65 % - 91,18% số lượng chủng mẫn cảm với enrofloxacin, norfloxacin và gentamycin, spectinomycin. Các chủng E. coli phân lập đã kháng lại tetramycine (80,88%), streptomycin (77,94%), oxacillin (66,18%) và erythromycin (42,65%). Các chủng vi khuẩn đều có độc lực rất cao, gây chết 100% động vật thí nghiệm trong vòng 8 - 48 giờ sau khi tiêm, Tóm tắt tiếng anh, This study was carried out to determine the prevalence of aerobic bacteria total and E. coli in pork selling in some markets of Dien Bien province. A total of 90 pork samples collected randomly at 3 local markets in Dien Bien province from June, 2016 to April, 2017 were analysed to determine the bacterial contamination in pork. The studied result showed that 98.89% of samples were positive with aerobic bacteria total. Of which, 48.89% of samples were contaminated with aerobic bacteria total over the limited level
75.56% of samples were contaminated with E. coli. Of which, 30 - 53.33% of samples were contaminated with E. coli over the permitted level of food safety, with the bacterial numbers ranging from 1.03x102 CFU/g to 5.23x102 CFU/g. The highest infection rate and intensity of E. coli in pork were in the central market. The prevalence of E. coli in pork was gradually increased and proportional to the time after slaughter and also increased in summer - autumn rather than in winter - spring. The isolated E. coli strains carried all the bio-chemistry characteristics of the E.coli bacteria and caused poison for human. The antibiotic susceptibility test of 68 E. coli strains showed that 67.65% - 91.18% of the isolated strains were susceptible to enrofloxacine, norfloxacine, gentamycine and spectinomycine. The isolated E. coli strains were strongly resistant to tetramycine (80.88%), streptomycin (77.94%), oxacillin (66.18%) and erythromycin (42.65%). All (100%) of the isolated E. coli strains were virulent, killing the experimental guinea pigs within 8 to 48 hours.

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