Ứng dụng phương pháp phả hệ phân tử để định danh mẫu nấm Cordyceps ninchukispora thu thập ở LangBiang, Đà Lạt, Lâm Đồng

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Tác giả: Nguyễn Văn Giang, Huệ Thiều Hồng, Phạm Thị Thúy Ngọc, Trương Bình Nguyên, Nguyễn Thị Ngọc Thảo, Thuận Lao Đức, Lê Huyền Ái Thúy

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ, 2024

Mô tả vật lý: tr.14-20

Bộ sưu tập: Báo, Tạp chí

ID: 418361

Thêm vào giỏ Liên kết toàn văn

Nhóm nghiên cứu của Trường Đại học Đà Lạt thuộc tỉnh Lâm Đồng, Việt Nam đã thu thập được mẫu nấm ký sinh từ dãy núi Lang Biang trong chuyến giám sát của nhóm, xác định hình thái ban đầu của loài này là Cordyceps ninchukispora có vật chủ là Beilschmiedia erythrophloia Hay. DNA đã được chiết tách bằng phương pháp Phenol/Chloroform (pH = 8), sau đó tiến hành thành PCR và giải trình tự của các vùng gen mục tiêu bao gồm ITS, nrSSU, nrLSU, Tef, Rpb1. Tạo cây phát sinh phân tử bằng việc sử dụng các phương pháp Neighbor joining, Maximum likelihood và Maximum parsimony nhằm xác định mối quan hệ giữa Cordyceps ninchukispora và các trình tự tham chiếu. Chúng tôi đã khuếch đại được các vùng gen mục tiêu, đồng thời tiến hành giải trình tự và đã xây dựng một bộ dữ liệu bao gồm 51, 51, 47, 35 và 51 trình tự của ITS, nrSSU, nrLSU, Tef, Rpb1. Các trình tự này đại diện cho nhiều chi thuộc các họ Ophiocordycipitaceae, Clavicipitaceae, Cordycipitaceae và 02 trình tự thuộc họ Glomerellaceae. Kết quả phân tích phát sinh phân tử đã xác định rằng mẫu nấm thu thập thuộc về chi Cordyceps và tạo thành đơn nhóm huyết thống (monophyletic group) với loài tham chiếu Cordyceps ninchukispora, với giá trị bootstrap được hỗ trợ cao. Tóm lại, đánh giá phát sinh loài dựa trên bộ dữ liệu đa gen đã hỗ trợ mạnh mẽ cho việc xác định mẫu thu thập được là Cordyceps ninchukispora.The research team from Dalat University, Lam Dong, Vietnam, collected entomopathogenic fungi samples from the Lang Biang mountain range during their routine survey. The initial morphological identification of this species indicated it as Cordyceps ninchukispora, with Beilschmiedia erythrophloia Hay. as the host. DNA was extracted using the Phenol/Chloroform method (pH = 8) and conducted PCR experiments and sequenced the target gene regions, including ITS, nrSSU, nrLSU, Tef, and Rpb1. Molecular phylogenetic trees were constructed using Neighbor-joining, Maximum likelihood, and Maximum parsimony methods to determine the relationship between Cordyceps ninchukispora and reference sequences. We successfully amplified and sequenced the target genes, resulting in a dataset containing 52, 52, 48, 39, and 52 sequences for ITS, nrSSU, nrLSU, Tef, and Rpb1. These sequences represented various genera within the families Ophiocordycipitaceae, Clavicipitaceae, Cordycipitaceae, and two sequences from the family Glomerellaceae. The results of the molecular phylogenetic analysis showed that the collected fungal sample belonged to the Cordyceps genus and formed a monophyletic group with the reference species Cordyceps ninchukispora, with high bootstrap support. In conclusion, the multigene dataset-based phylogenetic analysis strongly supported the identification of the collected sample as Cordyceps ninchukispora.

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