Betulinic acid and its derivatives display diverse bioactivities, such as anti-inflammatory, antitumor, anti-inflammatory, antibacterial, antimalarial, anticancer, and anti-HIV activities. Betulinic acid possesses three sites that are the most flexible toderivatization, including the C-3 hydroxyl, C-20 alkene, and C-28 carboxylic acid positions. This study aims to design and synthesis of a C-3 betulinic acid derivatives.Betulinic derivatives were synthesized by using classical esterification or ester - ester exchange reaction. The target product was isolated by using column chromatography. Its structure was determined by combining spectral analysis and comparison with related data. A new hybrid compound (compound 1c) was successfully synthesized from betulinic acid (BA) and lamivudine (3TC).