Khả năng phân hủy rơm rạ của các chủng xạ khuẩn thu thập ở đồng bằng sông Cửu Long

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Tác giả: Chí Thật Hồ, Mai Hoàng Duy và Lê Minh Tường Phạm

Ngôn ngữ: Vie

Ký hiệu phân loại: 631 Specific techniques; apparatus, equipment, materials

Thông tin xuất bản: Nông nghiệp và Phát triển nông thôn, 2020

Mô tả vật lý: 44 - 51

Bộ sưu tập: Metadata

ID: 419276

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Nghiên cứu được thực hiện tại Bộ môn Bảo vệ thực vật, Trường Đại học Cần Thơ nhằm tìm ra chủng xạ khuẩn có khả năng phân hủy rơm rạ. Khả năng phân giải cellulose của 22 chủng xạ khuẩn được thực hiện hoàn toàn ngẫu nhiên với 4 lần lặp lại. Kết quả cho thấy 7 chủng xạ khuẩn BT-VL5.4, PLBL16, TĐ-ST8, BT-VL3, CL2-ĐT34, LM-HG6 và LV-ĐT26 có khả năng phân giải cellulose cao thể hiện qua bán kính vòng phân giải lớn hơn 20,00 mm và kéo dài đến thời điểm 9 ngày sau khi cấy. Khả năng tiết enzyme cellulase của 7 chủng xạ khuẩn trên được thực hiện với 4 lần lặp lại. Kết quả cho thấy 3 chủng BT-VL5.4, PL-BL16 và LM-HG6 có khả năng tiết enzyme cellulase cao với hàm lượng enzyme tiết ra lần lượt là 0,117 IU/ml
0,098 IU/ml và 0,087 IU/ml ở thời điểm 9 ngày sau khi bố trí thí nghiệm. Khả năng phân hủy rơm rạ của 3 chủng xạ khuẩn (BT-VL5.4, PL-BL16 và LM-HG6) cũng được thực hiện trong điều kiện phòng thí nghiệm với 4 lần lặp lại. Kết quả cho thấy, 2 chủng BT-VL5.4 và PL-BL16 có khả năng phân hủy rơm rạ cao với khối lượng rơm rạ mất đi lần lượt là 0,841 g và 0,728 g và khối lượng tro còn lại sau khi xử lý nhiệt thấp lần lượt là 0,265 g và 0,288 g và khác biệt có ý nghĩa thống kê so với nghiệm thức đối chứng., Tóm tắt tiếng anh, The study was conducted at Plant Protection Department, Can Tho University to screen actinomycetes isolates able to ability in degrading straw. Cellulose degradation potential of 22 studied actinomycetes isolated were arranged completely randomized design with 4 replications. The results showed that 7 studied actinomycetes isolates, BT-VL5.4, PL-BL16, TĐ-ST8, BT-VL3, CL2-ĐT34, LM-HG6 and LV-ĐT26 able to high ability in degrading cellulose based on cellulolytic activity as the cellulose lyses halo radius of 20 mm higher and lasted at 9 days after testing. Evaluation the production of cellulase of 7 studied actinomycetes isolates with 4 replications show that BTVL5.4, PL-BL16 and LM-HG6 were the best with production of cellulase reaches 0.117 IU/ml
0.098 IU/ml and 0.087 IU/ml at 9 days after testing. The ability in degrading straw of 3 studied actinomycetes isolated (BT-VL5.4, PLBL16 and LM-HG6) were conducted at Plant Protection Department with 4 replications. The results showed that 2 studied actinomycetes isolates (BTVL5.4, PL-BL16) were able to high ability in degrading straw with weight loss of straw. They were 0.841 and 0.728 g respectively and the remaining ash weight after the low heat treatment were 0.265 g and 0.288 g respectively and significantly different from the control treatment.

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