Soil contamination with petroleum hydrocarbons is a major environmental concern. The bioremediation of these soils can be restricted because of the entry of potential microbial degraders into the viable but nonculturable (VBNC) state under unfavorable environmental conditions. Resuscitation agents can stimulate the resuscitation and growth of the bacterial population, improving their degradation capabilities. This study evaluated the efficacy of resuscitation-promoting factor (Rpf) in enhancing the biodegradation of total petroleum hydrocarbons (TPHs) in crude oil-contaminated soils, with a focus on both indigenous and augmented bacterial communities without prior resuscitation. Unlike earlier studies that focused solely on Rpf-treated populations, this study investigated the simultaneous application of Rpf and traditional biostimulation and bioaugmentation processes. Additionally, this work is the first to compare Rpf with other resuscitation agents, including supernatant Rpf (SRpf) and Micrococcus luteus. The results indicated that Rpf-supplemented biostimulation (BS+Rpf) and bioaugmentation (BAS+Rpf) processes achieved 67% and 75% degradation of 31,408 mg kg