KHẢO SÁT ẢNH HƯỞNG CỦA CÁC NGUỒN CARBON VÀ NITROGEN KHÁC NHAU Ở MÔ HÌNH IN VITRO LÊN KHẢ NĂNG TỔNG HỢP NHỰA SINH HỌC POLY-β- HYDROXYBUTYRATE (PHB) CỦA DÒNG VI KHUẨN Rhizobium gallicum M40.1=SURVEYING THE IN VITRO EFFECT OF DIFFERENT CARBON AND NITROGEN SOURCES ON POLY-β-HYDROXYBUTYRATE (PHB) BIOPLASTIC SYNTHESIS CAPACITY OF Rhizobium gallicum M40.1

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Tác giả: Thành Luân Nguyễn, Mỹ Hiếu Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Công nghệ và Thực phẩm, 2019

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Bộ sưu tập: Metadata

ID: 441268

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Nghiên cứu này nhằm mục đích khảo sát về ảnh hưởng của các nguồn carbon và nitrogen khác nhau ở mô hình in vitro lên khả năng tổng hợp PHB của loài vi khuẩn Rhizobium gallicum M40.1 trong môi trường Yeast Extract Manitol Medium (YEMM) lỏng. Các nguồn carbon như mannitol, sucrose, mật rỉ và tinh bột là các nguồn carbon dùng để khảo sát. NH4Cl, (NH42SO4, glycine, cao nấm men và TPY (tryptone yeast extract peptone với tỷ lệ 111) là các nguồn nitrogen được thử nghiệm. Vi khuẩn Rhizobium gallicum M40.1 được sử dụng thử nghiệm trong điều kiện nghiên cứu in vitro với hàm lượng nhựa PHB được tổng hợp ở nghiệm thức bổ sung sucrose có kết quả cao hơn so với các nghiệm thức có chứa nguồn carbon khác tương ứng 0,127 g/L PHB. Hiệu suất tích lũy nhựa của vi khuẩn được đánh giá đạt 41,06% trong điều kiện nuôi cấy lỏng trên máy lắc với tốc độ 150 vòng/phút, ở nhiệt độ thí nghiệm 30 °C trong 60 giờ tăng sinh. Kết quả khảo sát ảnh hưởng của các nguồn nitrogen khác nhau cho thấy các nghiệm thức bổ sung nguồn NH4khác nhau ở các nguồn NH4Cl, (NH42SO4, glycine và hỗn hợp Trypton Peptone Yeast Extract (TPY) với NH4Cl giúp sản sinh nhựa tốt nhất với 0,029 0,013 g/L tương ứng 61,09%. Môi trường YESUM có bổ sung NH4Cl ở nồng độ 1% đã giúp vi khuẩn Rhizobium gallicum M40.1 tổng hợp PHB cao hơn gấp 3 lần so với nghiệm thức đối chứng không bổ sung nitrogen với cùng nồng độ trong điều kiện lên men gián đoạn.Từ khóa Nhựa sinh học, nguồn carbon, nguồn nitrogen hữu cơ, PHB, Rhizobium gallicum M40.1The aim of this study was to survey the in vitro effect of different organic carbon and nitrogen sources on the PHB bio-plastic production synthesized by Rhizobium gallicum type M40.1 in liquid yeast extract manitol medium (YEMM). Manitol, sucrose, molasses and starch were used as tested organic carbon sources. Addionally, NH4Cl, (NH42SO4, glycine, yeast extract and TPY (included trypton, yeast extract and peptone with the ratio of 111) were considered as nitrogen sources. Rhizobium gallicum type M40.1 with a function of PHB bio-plastic synthesis was isolated f-rom soil and the experiments were conducted under in vitro laboratory conditions. The results showed that the amount of synthesized PHB bio-plastic by Rhizobium gallicum type M40.1 in the treatment supplemented with sucrose as an organic carbon source was significantly higher than that in the others, with an amount of 0.127 g/L which obtained 41.06% PHB synthesized under continuous condition after 60 hours of incubation. The different results in nitrogen sources illiminated the NH4+ supplementation f-rom NH4Cl, (NH42SO4, glycine and the mixture of trypton peptone yeast extract (TPY) with NH4Cl contributed better PHB systhesis capacity with 0,029 0,013 g/L equal to 61,09%. The result of the effect of different nitrogen source supplement on PHB bio-plastic synthesizing capacity of Rhizobium gallicum type M40.1 in YEMM revealed that the larger amounts of synthesized PHB bio-plastic were found to be 3 times higher in all NH4Clcontaining nitrogensource treatments with concentration of 1% than that of the yeast extract powder treatment with the same concentration under discontinuous fermentation conditions. KeywordsBioplastic, nitrogen sourcesorganic carbon sources PHB, Rhizobium gallicum M40.1.

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