Bayberry (Myrica rubra Sieb. et Zucc.) kernel is a by-products of bayberry processing and are rich in oil. This study investigates the effects of different processing methods on bayberry kernel oil (BKO) yield, including cold pressing (CP), direct solvent (n-hexane) extraction, ultrasound-assisted n-hexane extraction (UAE), steam explosion pretreatment to assist n-hexane extraction, aqueous enzyme extraction, and supercritical fluid extraction (SFE). The acid value, peroxide value, fatty acid composition, bioactive components content, and the antioxidant activity of the extracted BKO with six methods were compared. The results showed that the oil yield of bayberry kernel extracted by different methods was between 30.95% and 49.49%, and the oil yield of UAE was the highest. BKO samples extracted by different methods had lower acid value (0.39-0.59 mg KOH/g) and peroxide value (3.97-5.84 meq O