In the current 4.0 context, applying information technology in teaching is a mandatory element in education. However, research on the application of digitizing image and video data in teaching Anatomy to nursing students is limited and has not been widely implemented in practice. In this study, we have designed a five-step process for building electronic learning materials to complement anatomy instruction, based on six principles. From there, we created a collection of anatomy learning resources including 8 films, 8 image files, and 8 sets of assessment questions corresponding to each of the human body’s organ systems. The results were evaluated and instructors and students who participated directly provided excellent feedback.