Nano titanium dioxide (TiO2) is an important material that has been extensively investigated due to its uniqueness. A large number of efforts have been made to synthesize nano titanium dioxide materials using different methods. In this work, nano TiO2 was successfully synthesized by the hydrothermal method under acidic-base conditions using commercial TiO2 powder. Morphological and size information about the synthesized material was determined using EDX and SEM analysis. EDX analysis provides the elemental composition of the synthesized product. Nitrogen adsorption-desorption was used to determine the surface area (Brunauer-Emmett-Teller (BET)), porosity, and capillary distribution of the material. The TiO2nanoparticles were tested for their antibacterial activity against human pathogens such as gram-negative Escherichia coli (E. coli) and gram-positive Staphylococcus aureus (S. aureus) using the paper ring diffusion method on agar medium, yielding excellent results.