Phosphoinositide-specific phospholipase C (PI-PLC) has been known as one of the key enzymes that involved in the phospholipid hydrolysis. However, the PI-PLC family in cassava has not been fully recorded. In this study, a comprehensive analysis of the PI-PLC family in cassava assembly has been performed based on various bioinformatics tools. Particularly, a total of seven members of the PI-PLC family has been identified and annotated in the cassava genome. By using the full-length protein sequence of each member of the PI-PLC family in cassava, the properties of these proteins, including the length, size, iso-electric point, instability index, aliphatic index and grand average of hydropathy were analyzed. More interestingly, the expression patterns of genes encoding the PI-PLC family in various major organs/tissues in different conditions were investigated. Taken together, this current study could provide a solid foundation for the PI-PLC family in cassava for further functional characterization towards the improvements of drought stress tolerance in cassava plants.