NGHIÊN CỨU SỰ DẬP TẮT HUỲNH QUANG CỦA RHODAMINE B PHỤ THUỘC VÀO BƯỚC SÓNG KÍCH THÍCH ĐỂ ĐỊNH LƯỢNG HẠT NANO BẠC THÔNG QUA HIỆU ỨNG NỘI DẬP TẮT

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Tác giả: Thu Trang Trần

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2024

Mô tả vật lý: tr.104 - 111

Bộ sưu tập: Báo, Tạp chí

ID: 462475

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Hạt nano bạc (Ag NPs) kích thước khoảng 9 nm đã được tổng hợp và nghiên cứu định lượng thông qua sự dập tắt huỳnh quang của phân tử màu rhodamine B (RhB). Một hệ các dung dịch bao gồm RhB và Ag NPs với nồng độ Ag NPs thay đổi từ 0,09 pM đến 0,98 pM đã được chuẩn bị và nghiên cứu tính chất quang bằng phổ hấp thụ và phổ huỳnh quang. Sự dập tắt huỳnh quang của RhB đã quan sát được trong các hệ dụng dịch Ag NPs và RhB. Điều kiện đo phổ huỳnh quang đã được tối ưu để thu được hiệu suất dập tắt huỳnh quang cao nhất. Qua khảo sát sự dập tắt huỳnh quang phụ thuộc vào các bước sóng kích thích khác nhau đã cho thấy hiệu suất dập tắt huỳnh quang xảy ra cao nhất khi kích thích ở bước sóng 400 nm. Ở điều kiện kích thích 400 nm, nồng độ Ag NPs thấp nhất để có thể phát hiện được đạt tới 0,09 pM. Đây là giới hạn phát hiện Ag NPs tốt so với các công bố trước. Cơ chế của sự dập tắt huỳnh quang được chỉ ra là do hiện tượng nội dập tắt (Inner filter effect).Silver nanoparticles (Ag NPs) with a size of around 9 nm were fabricated and quantified through fluorescence quenching of rhodamine B (RhB). A series of solutions composed of RhB with various concentrations of Ag NPs ranging from 0.09 pM to 0.98 pM were prepared, and optical properties were investigated by absorption and fluorescence spectroscopies. The fluorescence quenching of RhB was observed for the mixing solution of RhB and Ag NPs. The fluorescence measurement condition was optimized by controlling excitation wavelengths to obtain the highest efficiency of fluorescence quenching. The examination of the fluorescence quenching depending on excitation wavelengths indicated that under the excitation wavelength of 400 nm, the highest efficiency of fluorescence quenching of RhB was achieved. At this optimized condition, the lowest concentration of Ag NPs that could be quantified reaches 0.09 pM. It shows a good limitation in the detection of Ag NPs in comparison with the previous works. The mechanism of fluorescence quenching of RhB with the presence of Ag NPs has been revealed as the result of the inner filter effect.

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