The application of beneficial microorganisms as well as their secondary products in the livestock, agriculture, and food industry supply chains for sustainable green production is a developing trend. The research direction of selecting multi-activity strains to enhance the effectiveness of practical applications has been receiving attention. This study aims to select endophytic fungal strains on Huperzia javanica plant in Vietnam that have the ability to produce multi-extracellular enzymes and multi-resistance to pathogenic microorganisms by determining enzyme activity and testing antimicrobial activity. The results showed the following: (1) All 9 strains have the ability to produce 1 to 5 types of enzymes and inhibit 1 to 5 pathogenic microorganisms with potential activities
Strains TLC11 and TLC9 produce 4÷5 enzymes (cellulase, lipase, protease, phosphatase and β-galactosidase) with the highest hydrolysis zone diameters of 22÷25 mm (protease) and 20÷23 mm (lipase)
(2) Strain TLC13 inhibits all 5 tested microbial strains Escherichia coli, Staphylococcus aureus, Candida albican, Bacillus cereus, Pseudomonas aerigunosa) with the highest activity against 3 species B. cereus (24±1.2 mm), P. aerigunosa (26±1.1 mm) and C. albican (36±1.5 mm)
Strains TLC10 and TLC19 are resistant against 4/5 tested microorganisms except S. aureus (TLC10) and E. coli (TLC19). These strains could be the potential sources for further in-depth research aiming to expand their applications in sustainable agriculture, aquaculture, and industry production fields.