A rubrerythrin locus of Clostridioides difficile encodes enzymes that efficiently detoxify reactive oxygen species.

• Tác giả: Silvia Dittmann, Simon Keweloh, Robert Knop, Johanna Pukall, Susanne Sievers, Daniela Zühlke
• Ngôn ngữ: eng
• Ký hiệu phân loại: 551.576 Clouds
• Thông tin xuất bản: England : Anaerobe , 2025
• Bộ sưu tập: NCBI
• ID: 462812

Tóm tắt

OBJECTIVES: The microaerophilic conditions in the large intestine and reactive oxygen species (ROS) produced by the immune system represent a challenge for the strictly anaerobic pathogen Clostridioides difficile, which protects itself by a variety of oxidative stress proteins. Four of these are encoded in an operon that has been implicated in the detoxification of H METHODS: Recombinant proteins were overexpressed in C. difficile and purified anaerobically by affinity chromatography. The H RESULTS: Proposed roles of the investigated proteins in the detoxification pathways of ROS could partially be demonstrated. Specifically, Rbr and glutamate dehydrogenase synergistically detoxify H CONCLUSIONS: The investigated gene locus codes for an oxidative stress operon whose members have the potential to neutralize O