In recent years, the infection rate of avian reovirus (ARV) in chicks has been continuously increasing, seriously endangering the healthy development of China's chicken farming industry. However, the pathogenicity of this virus in immune organs has been less studied. In this study, an ARV strain (Chicken/XJ/China/2022/01/16) was isolated from arthritic broiler chicks in Xinjiang province and whole genome sequencing of the ARV strain was performed using next generation sequencing (NGS) technology, and sequence analysis showed that the strain was a type V variant of the ARV gene. In order to further understand the pathogenicity of this strain in terms of immune organs, we randomized 120 healthy 1-day-old broilers equally into 3 groups (oral, footpad inoculation and control), where 2 experimental groups were inoculated with 0.2 mL of ARV (TCID