Marked differences in thyroxine (T4) metabolism following in vitro exposure of Wistar rat and human hepatocytes to several reference CAR/PXR nuclear receptor activators.

• Tác giả: Audrey Baze, Liliia Horbal, Betty Ory, Lysiane Richert, Helen Tinwell
• Ngôn ngữ: eng
• Ký hiệu phân loại: 133.535 Mars
• Thông tin xuất bản: England : Toxicology in vitro : an international journal published in association with BIBRA , 2025
• Bộ sưu tập: NCBI
• ID: 463605

Tóm tắt

 Our study builds upon previous findings (Baze et al., 2024) by investigating species differences in thyroxine (T4) metabolism regulation by CAR/PXR activators using cryopreserved primary Wistar rat hepatocytes (PRH) and human hepatocytes (PHH) in 2D-sandwich over a 7-day treatment period. Daily exposure of PRH to phenobarbital, 5-Pregnen-3β-ol-20-one-16α‑carbonitrile (PCN) or dexamethasone increased T4 clearance over the last 24 h exposure (up to 60 %, 79 % and 67 % over control, respectively) and secretion of T4-glucuronide (T4-G
  up to 463, 661 and 545 pmol/10