Structural pathway for PI3-kinase regulation by VPS15 in autophagy.

• Tác giả: Ainara Claveras Cabezudo, Minghao Chen, Annan S I Cook, Samantha N Garcia, Gerhard Hummer, James H Hurley, Anthony T Iavarone, Grace Khuu, Michael Lazarou, Thanh N Nguyen, Shanlin Rao, Xuefeng Ren, Mingxuan Yang
• Ngôn ngữ: eng
• Thông tin xuất bản: United States : Science (New York, N.Y.) , 2025
• Bộ sưu tập: NCBI
• ID: 463676

Tóm tắt

The class III phosphatidylinositol-3 kinase complexes I and II (PI3KC3-C1 and -C2) have vital roles in macroautophagy and endosomal maturation, respectively. We elucidated a structural pathway of enzyme activation through cryo-EM analysis of PI3KC3-C1. The inactive conformation of the VPS15 pseudokinase stabilizes the inactive conformation, sequestering its