Electrochemical biosensors have been extensively researched and employed across diverse fields from environmental monitoring to clinical diagnostics. Detecting biomarkers like saliva pH and glucose are crucial indicators of the health and well-being of animals and opens the door for development of new non-invasive calf health measurements. Herein, we introduce a highly sensitive and stable electrochemical sensor for detection of pH and glucose in artificial and calf saliva. Pristine gold electrodes were employed for pH measurement using the voltage where the minimum of the gold oxide reduction peak occurred as a pH indicator. For glucose sensing, we utilized an effective in-situ pH control method enabled by interdigitated microelectrodes (IDEs) to optimize pH for accurate detection of glucose in artificial and calf saliva. Glucose oxidase (GOx) was first immobilized onto a platinum black modified gold IDE array through an electrodeposition process, which involved a mixture of o-phenylenediamine (o-PD) and β-cyclodextrin (β-CD). The enzymatic based glucose sensor showed an exceptional sensitivity of -0.46 nA mM