This study aimed to validate a tool for assessing vaccine literacy (VL) in adult vaccination under the Chinese context. A pilot study on adult's VL was conducted in 2023 in Huzhou city of Zhejiang province, China. A self-designed questionnaire, specific for the self-assessment of three domains (functional, interactive, and critical), as well as the vaccination knowledge and acceptance was applied. Validation process of the questionnaire was conducted through the investigation of a panel of experts, principal component analysis (factors whose cumulative explanation proportion of variance >
80% were selected) and Cronbach's α coefficient test (>
0.7 was acceptable). Correlation coefficient analysis for VL domains, vaccination knowledge and acceptance were also implemented, and