BACKGROUND: Lung cancer is the most common malignant tumor in China. In 2016, more than 800,000 new cases of lung cancer were diagnosed in China. Squamous cell carcinoma of the lung, a type of non-small cell lung cancer (NSCLC), accounts for 25-30% of all lung cancer cases, and has an overall 5-year survival rate of about 32.53%, lower than adenocarcinoma for which there have been far more therapeutic advances in the last few decades. The purpose of this study was to explore the mechanisms of the disease and to identify potential prognostic biomarkers. METHODS: This study analyzed lung squamous cell carcinoma of the lung tissues and paraneoplastic tissues to identify differentially expressed genes (DEGs). We conducted a Gene Set Enrichment Analysis and prognostic analysis by constructing competing endogenous RNA (ceRNA) networks
we performed a correlation analysis of the target genes and verified the targeting relationship of the ceRNA by cellular assays. We assessed the effects of the target genes on tumor cell proliferation, invasion and apoptosis by Cell Counting Kit-8 (CCK-8) assays, invasion assays, and caspase 3/7 assays, respectively. RESULTS: We identified 4,039 downregulated genes and 1,924 upregulated genes. The p53 pathway, cell-cycle pathway and mismatch-repair (MMR) pathway were activated, while the mitogen-activated protein kinase pathway was inhibited. Two ceRNA networks centered on the long non-coding RNAs (lncRNAs) MAGI2-AS3 and LINC01089 were constructed.