BACKGROUND: Burnout syndrome is a significant issue among healthcare professionals worldwide, marked by depersonalization, emotional exhaustion, and a reduced sense of personal achievement. This psychological and physical burden profoundly affects healthcare professionals' quality of care and overall well-being. In Somalia, where the healthcare system faces numerous challenges, the escalating demand for medical services and inadequate resources, coupled with overwhelming workloads, long hours, and high-stress levels, make healthcare providers particularly vulnerable to burnout syndrome. This, in turn, affects both the mental health of healthcare personnel and the quality of care they provide. AIM: To examine the prevalence and determinants of burnout syndrome among healthcare practitioners in Mogadishu, Somalia. METHODS: This cross-sectional prospective study was performed among 246 healthcare providers employed at a tertiary care hospital in Mogadishu, Somalia, who were recruited RESULTS: Among the participants, 24% (95%CI: 18.8%-29.8%) exhibited symptoms of burnout syndrome. Factors associated with burnout included female gender (AOR = 6.60
95%CI: 2.29-19.04), being married (AOR = 3.07
95%CI: 1.14-8.28), being divorced or widowed (AOR = 5.84
95%CI: 1.35-25.35), working more than 7 night shifts (AOR = 3.19
95%CI: 1.30-7.82), having less than 5 years of job experience (AOR = 5.28
95%CI: 1.29-21.65), experiencing poor sleep quality (AOR = 5.29
95%CI: 1.88-14.89), and exhibiting depressive (AOR = 4.46
95%CI: 1.59-12.53) and anxiety symptoms (AOR = 7.34
95%CI: 2.49-21.60). CONCLUSION: This study found that nearly one in four healthcare professionals suffers from burnout syndrome. Improving sleep quality, monitoring, and providing mental health support could enhance their well-being and patient care.