Một số đặc điểm sinh học của virus dịch tả lợn nhược độc chủng C được thích nghi trên tế bào PK15 tại Công ty HanVet = Characteristics of attenuated classical swine fever virus - C strain adapted on PK15 cell line at Hanvet company

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Tác giả: Nguyễn Cảm, Hữu Vũ Nguyễn, Thanh Ba Nguyễn, Thị Bích Nguyễn, Thu Trang Nguyễn, Văn Phúc Nguyễn, Văn Tâm Nguyễn, Văn Khánh Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2019

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 470606

Thêm vào giỏ

Classical swine fever virus, C strain was adapted on PK15 cell line at Hanvet Company. The biological characteristics of cell-adapted virus showed that its identity and purity met the required standards. The growth kinetic of virus was very stable, the highest viral titer was 106.5 TCID50/ml at 72h post-infection. The cell-adapted virus retained the ability to induce fever in the experimental rabbits
the minimum infective dose in the experimental rabbits was 10-5/ml. The pigs were safe when inoculation with dose of 106.5 TCID50/pig. The inoculated pigs with 101
102
103 and 104 TCID50 of cell-adapted virus induced good immune response and they were completely protected when challenging with 103 MLD50 virulent CSFV. Virus nhược độc dịch tả lợn, chủng C đã được thích nghi trên tế bào PK15 tại công ty Hanvet. Sau khi thích nghi, virus đã được kiểm tra các đặc tính sinh học của giống. Kết quả kiểm tra cho thấy chủng virus đạt các tiêu chuẩn về nhận dạng, thuần khiết. Đường cong sinh trưởng của virus trên tế bào ổn định, hiệu giá virus cao nhất đạt 106,5 TCID50/ml tại 72 giờ sau khi gây nhiễm. Chủng virus thích nghi trên tế bào vẫn giữ được đặc tính gây sốt thỏ, liều gây nhiễm tối thiểu trên thỏ là 10-5/ml. Virus an toàn khi tiêm cho lợn với liều 106,5 TCID50/lợn. Gây miễn dịch cho lợn bằng chủng virus dịch tả lợn thích nghi trên tế bào với các liều miễn dịch 101, 102, 103 và 104 TCID50, lợn đều có đáp ứng miễn dịch tốt và được bảo vệ hoàn toàn khi thử thách với 103 MLD50 virus dịch tả lợn cường độc.

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