INTRODUCTION: Wall-associated kinases (WAKs) are pivotal in linking plant cell walls to intracellular signaling networks, thereby playing essential roles in plant growth, development, and stress responses. METHODS: The bioinformatics analysis was employed to identify WAK genes in tobacco. The expression levels of RESULTS: We conducted a comprehensive genome-wide identification and analysis of the DISCUSSION: This study represents the first genome-wide analysis of the WAK gene family in