URAdime - a tool for analyzing primer sequences in sequencing data to identify dimers and super-amplicons.

• Tác giả: Jason D Limberis, John Z Metcalfe
• Ngôn ngữ: eng
• Ký hiệu phân loại: 070.194 Radio
• Thông tin xuất bản: United States : bioRxiv : the preprint server for biology , 2025
• Bộ sưu tập: NCBI
• ID: 471056

Tóm tắt

Targeted sequencing of multiplex PCR amplicons is commonplace in research laboratories and clinical diagnostics. There are numerous tools for the a priori optimization of primers and reactions, but no tools to detect specific problematic primers post hoc. We developed URAdime, a tool for analyzing primer sequences in sequencing data to identify dimers and super-amplicons. We show that it successfully detects these unwanted amplicons and accurately attributes their generation to specific primers.