Electrochemically modulated single-molecule localization microscopy for

• Tác giả: Dehong Hu, Chenghong Lei
• Ngôn ngữ: eng
• Ký hiệu phân loại: 005.113 Structured programming
• Thông tin xuất bản: Germany : Nanophotonics (Berlin, Germany) , 2025
• Bộ sưu tập: NCBI
• ID: 471613

Tóm tắt

A new concept of electrochemically modulated single-molecule localization super-resolution imaging is developed. Applications of single-molecule localization super-resolution microscopy have been limited due to insufficient availability of qualified fluorophores with favorable low duty cycles. The key for the new concept is that the "On" state of a redox-active fluorophore with unfavorable high duty cycle could be driven to "Off" state by electrochemical potential modulation and thus become available for single-molecule localization imaging. The new concept was carried out using redox-active cresyl violet with unfavorable high duty cycle as a model fluorophore by synchronizing electrochemical potential scanning with a single-molecule localization microscope. The two cytoskeletal protein structures, the microtubules from porcine brain and the actins from rabbit muscle, were selected as the model target structures for the conceptual imaging