Haemonchus contortus is a pathogenic nematode that infects small ruminants. Chemotherapy is the main treatment for these parasitic infections, but the rapid rise of drug resistance calls for the development of new anthelmintics. To support this, optimizing screening assays is vital for identifying new drugs. The exsheathed L3 (xL3) stage of H. contortus is often used in in vitro evaluations
however, it has been observed that it is less sensitive than the adult stage, possibly due to enhanced detoxification pathways. To explore this hypothesis, inhibitors of xenobiotic detoxification pathways were tested on the activity (IC