THỬ HOẠT TÍNH SINH HỌC VÀ XÁC ĐỊNH HÀM LƯỢNG JATRORRHIZINE BẰNG PHƯƠNG PHÁP LC/MS CÓ TRONG THÂN CÂY MẬT GẤU (MAHONIA NEPAULENSIS DC.) Ở ĐẠI TỪ - THÁI NGUYÊN = TESTING BIOACTIVE AND DETERMINATION OF JATRORRHIZINE CONTENT BY USING LC/MS METHOD IN THE STEM OF MAHONIA NEPAULENSIS DC. IN DAI TU, THAI NGUYEN

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Tác giả: Thị Giang Lê, Thị Mỹ Ninh Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2020

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 472616

Thêm vào giỏ

Cây mật gấu (Mahonia nepaulensis DClà một trong nhiều cây thuốc quý ở Việt Nam. Trong nội dung nghiên cứu này, nhóm tác giả đã tiến hành khảo sát các lớp chất, thử hoạt tính sinh học và xác định hàm lượng jatrorrhizine có trong thân cây mật gấu. Các phương pháp nghiên cứu được sử dụng bao gồm phương pháp ngâm chiết để thu dịch chiết, phương pháp hóa học để khảo sát các lớp chất từ dịch chiết cây mật gấu, phương pháp thử hoạt tính sinh học, phương pháp xây dựng đường chuẩn để xác định hàm lượng phần trăm jatrorrhizineKết quả cho thấy trong cặn chiết ethanol của thân cây mật gấu có alkaloids, steroids, coumarin, cardiac glycosides. Cặn chiết ethanol có khả năng kháng yếu đối với chủng nấm mốc Fusarium oxysporum (200 µg/mL), cặn n-butanol có khả năng kháng yếu đối với chủng vi khuẩn Staphylococcus aureus (149,3 µg/mL). Hàm lượng jatrorrhizine có trong cây mật gấu là 0,682203%. Việc xác định hàm lượng jatrorrhirine có trong thân cây mật gấu có ý nghĩa quan trọng trong việc mở ra hướng nghiên cứu thuốc chữa bệnh tiểu đường, bệnh vẩy nến và bệnh Alzheimer. Mahonia nepaulensis DC. is one of many valuable medicinal herbs in Vietnam. In this study, we have conducted a survey of substance layers, tested bioactive and determined of jatrorrhizine content in the stem of M. nepaulensis. The research methods used include extraction method to obtain the extract, chemical methods for surveying layers of the extracted liquid of the M. nepaulensis, bioactive test method and a standardized method for calibration line to determine jatrorrhizine content was used in this study. Phytochemical analysis showed the presence of alkaloids, steroids, coumarin and cardiac glycosides in the ethanol extract of the stem of this plant. The ethanol extract showed weak resistance to the Fusarium oxysporum (200 µg/mL), and n-butanol showed weak resistance to the Staphylococcus aureus (149.3 µg/mL). The content of jatrorrhirne in the M. nepaulensis is 0.682203%. The determination of the jatrorrhirne content in the stem of this species is an important significance in researches for the treatment of diabetes, psoriasis and alzheimer.

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