Những biến đổi sinh lý, hóa sinh của cây Phong lan Phi điệp tím (Dendrobium anosmum Lindl.) trong quá trình luyện ex vitro=Physiological and biochemical changes of micropropagated Dendrobium anosmum Lindl. in ex vitro acclimatization process

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Tác giả: Phi Bằng Cao

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Phát triển Khoa học & Công nghệ: Chuyên san Khoa học Tự nhiên, 2018

Mô tả vật lý:

Bộ sưu tập: Báo, Tạp chí

ID: 472993

Thêm vào giỏ

Quá trình luyện ex vitro có ý nghĩa rất lớn đối với công nghệ vi nhân giống. Cây có nguồn gốc in vitro phải thích nghi rất nhanh chóng với sự thay đổi của môi trường. Công trình này có mục tiêu nghiên cứu một số biến đổi sinh lý, hóa sinh của cây Phong lan Phi điệp tím có nguồn gốc in vitro trong quá trình luyện ex vitro như các hàm lượng nước, chất khô, proline cũng như các sắc tố quang hợp (chlorophyll a, chlorophyll b và carotenoid), huỳnh quang chlorophyll và hoạt độ của một số enzyme chống oxy hóa (peroxidase và catalase). Kết quả nghiên cứu chỉ ra rằng hàm lượng nước giảm xuống trong cây ex vitro so với cây in vitro. Hàm lượng chlorophyll và carotenoid trong mô lá tăng theo quá trình luyện ex vitro. Khi các cây được chuyển khỏi môi trường in vitro, hiệu suất quang hóa cực đại của quang hệ II (Fv/Fm) giảm xuống ở những thời kì luyện sớm và chỉ phục hồi ở thời kì muộn của quá trình luyện cây. Hàm lượng proline và hoạt độ các enzyme chống oxy hóa tăng lên ở các thời kì khác nhau của quá trình luyện. Giá trị cực đại của hàm lượng proline và hoạt độ các enzyme được ghi nhận ở pha ex vitro đầu tiên, thời kì cây bị mất nhiều nước nhất. Những kết quả nghiên cứu này gợi ý rằng cây phong lan Phi điệp tím in vitro đã thích nghi với sự chuyển môi trường sống bằng cách phát triển những đáp ứng sinh lí của hệ thống quang hợp cũng như bộ máy chống oxi hóa.The ex vitro acclimatization process plays an important role in plant micropropagation. In vitro plantlets have to rapidly adapt to environmental changes. The current work aimed at assessing some physiological and biochemical changes of micropropagated Dendrobium anosmum Lindl. Plantlets during ex vitro acclimatization process, eg. contents of water (leaf relative water content), dry matter, proline and photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoid), chlorophyll fluorescence and antioxidant enzymes (peroxidase và catalase) activities. The analyzed results showed that water content decreased in acclimatized plantlets compared to in vitro ones. The chlorophylls and carotenoids contents of what were significantly higher in ex vitro plantlet leaves compared to the day 0 plantlets. The pigment contents were observed to increase during the ex vitro acclimatation process. When the plantlets were moved out of the in vitro medium, the maximum photochemical efficiency of photosystem II (Fv/Fm) significantly decreased at the early acclimatation points then restored at the end of acclimatation process. The content of proline and activities of antoxidant enzymes significantly increased with different periods of acclimatation process. The proline content and enzyme activities were recorded at the first ex vitro period when most water loss occurred in plantlets. These results suggest that Dendrobium anosmum Lindl in vitro plantlets have adapted to the transplantation by possesing some physiological responses of its photosynthetic system as well as its antioxidant machinery.

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