Điều chế exopolysaccharide sulfate từ nấm Ophiocordyceps sinensis và tác dụng kháng oxy hóa của chúng=Preparation of a sulfated exopolysaccharide (S-EPS) from Ophiocordyceps sinensis fungus and its antioxidant effects

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Tác giả: Minh Hiệp Đinh, Thư Huỳnh, Thị Thúy Hằng Lê, Thị Lài Nguyễn, Thị Xuân Phương Nguyễn, Minh Trang Trần

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Phát triển Khoa học & Công nghệ: Chuyên san Khoa học Tự nhiên, 2018

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Exopolysaccharide sulfate đã được biết đến làm cải thiện các hoạt tính sinh học. Expolysaccharide (EPS) sản xuất bởi nấm Ophiocordyceps sinensis là một nguồn dược chất tự nhiên. Do vậy, mục đích chính của nghiên cứu này là nâng cao hoạt tính sinh học của EPS thông qua sự thay đổi thành phần nhóm sulfate bằng phương pháp acid chlorosulfonic (CSA) và pyridin (Pyr). Điều kiện phù hợp cho phản ứng sulfate hóa được xác định, bao gồm tỷ lệ CSA/Pyr là 13 (v/v), và 6 giờ. S-EPS11 có độ thay thế cao nhất là 1,59, với thành phần đường tổng và sulfate lần lượt là 52,25% và 47,15%. Bên cạnh đó, phân tích FT-IR chứng minh S-EPS11 có sự hiện diện của dao động dãn C-O-S (ở 815 cm-1) và S=O (ở 1129 cm-1), trong khi EPS không hiện diện những dao động này. Đặc biệt, khả năng bắt gốc tự do OH• và ABTS• của S-EPS11 tăng lên đáng kể so với EPS không sulfate. Kết luận, chúng tôi đã tổng hợp thành công dẫn xuất exopolysaccharide sulfate từ nấm O. sinensis với hoạt tính kháng oxy hóa được cải thiện rõ rệt.Sulfated exopolysaccharides have been well-known to enhance biological activities. Exopolysaccharide (EPS) produced by Ophiocordyceps sinensis fungus is a source of natural compounds. The aim of our study is to improve the EPS biological activities by its sulfated modification using the chlorosulfonic acid (CSA)-pyridine (Pyr) method. The appropriate conditions of the sulfation reaction were explored, including CSA/Pyr ratio (v/v) of 13 and 6h. The degree of substitution (DS) of S-EPS11 was the highest (DS = 1.59). The total contents of polysaccharides and SO42- of S-EPS11 were 52.25% and 47.15%, respectively. Besides, the FT-IR spectra analysis indicated the presence of C-O-S (peak of 815 cm-1) and S=O (peak of 1129 cm-1) stretching vibrations, while the natural EPS did not appear. Importantly, OH• and ABTS• radical scavenging potential of S-EPS11 significantly increased compared with those of the natural EPS. Together, we successfully generated sulfated EPS extracted from O. sinensis fungus which enhanced antioxidant activities of natural EPS.

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