Khảo sát tác động của giá thể hydrogel màng ối đến sự phát triển của nang tiền hốc ở mô hình chuột=Evaluation of amniotic hydrogel’s impact on the development of murine pre-antral follicles in mouse model

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Tác giả: Thị Vĩ Tuyết Lê, Lê Bảo Hà Trần, Thị Kim Hằng Trần, Ngọc Lê Vân Trịnh, Thụy Anh Thư Võ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Phát triển Khoa học & Công nghệ: Chuyên san Khoa học Tự nhiên, 2018

Mô tả vật lý:

Bộ sưu tập: Báo, Tạp chí

ID: 473493

Thêm vào giỏ

Global average infertility rate is about 6–12%, and in Vietnam at around 7.7%. As a result, there is a high demand for treatment, especially for female infertility. In vitro maturation (IVM) was evaluated and proven to be the most popular and promising at the moment. In long-term cultivation, the follicle was observed to extend, therefore, the usage of a supporting frame is quite necessary to maintain follicle’s natural sphere structure as well as completing the mature process. Amniotic membrane is an avascular membrane, composed of collagen, fibronectin, nidogen, proteoglycan, containing a big number of growth – factors with antimicrobial, anti-inflammatory, low immunogenicity and viscoelasticity properties. Amniotic hydrogel owns structure formed with thin fibers to help preserve the main component as collagen, which can turn to gel form at 37 degree Celsius. With those properties, amniotic hydrogel showed high potential as a scaffold for the follicle. When amniotic hydrogel is used as a scaffold for cultivating of secondary follicle (100 – 130 μm), the size of oocyte and follicle increased after 12 days of culturing, along with the formation of antrum. The results demonstrated the possibility to use amniotic hydrogel as a scaffold for the development of the secondary follicle.Trên thế giới, tỷ lệ vô sinh ở trong khoảng 6 – 12 %
tại Việt Nam, tỷ lệ vô sinh này là 7,7 %. Vì vậy, nhu cầu điều trị vô sinh là rất lớn và đặc biệt phức tạp đối với vô sinh nữ. Nuôi trưởng thành noãn trong ống nghiệm (IVM) được đánh giá là kỹ thuật hỗ trợ thụ tinh trong ống nghiệm (TTTON) phổ biến và tiềm năng hiện nay. Khi nuôi trong thời gian dài, nang noãn thường có hiện tượng bị trải rộng, việc sử dụng thêm khung nâng đỡ trong quá trình nuôi nang giúp duy trì cấu trúc hình cầu tự nhiên, từ đó giúp nang noãn phát triển hoàn thiện. Màng ối là một màng vô mạch, có thành phần chính là collagen, fibronectin, nidogen, proteoglycan, … có chứa nhiều nhân tố tăng trưởng, có đặc tính kháng khuẩn, kháng viêm, tính sinh miễn dịch thấp, có độ đàn nhớt tự nhiên. Hydrogel màng ối có cấu trúc mạng lưới được hình thành từ các sợi dài mảnh, bảo toàn được thành phần chính là collagen, có thể chuyển pha và tạo thành dạng gel. Từ những đặc điểm trên, hydrogel màng ối thể hiện tiềm năng trong việc sử dụng làm khung nâng đỡ nuôi nang noãn. Khi sử dụng hydrogel màng ối làm khung nâng đỡ nuôi nang thứ cấp (100 – 130 μm), kích thước noãn và nang tăng sau 12 ngày nuôi, bên cạnh đó, còn có sự hình thành hốc. Những kết quả thu được cho thấy có thể sử dụng hydrogel màng ối làm khung nâng đỡ cho nang noãn thứ cấp phát triển.

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