OBJECTIVES: The progression of recruitment of motor unit potentials (MUPs) during increasing voluntary contraction can provide important information about the motor units (MUs) innervating a muscle. Here, we described a method to quantitate the recruitment level of the intramuscular electromyographic (iEMG) signal during an increasing force level. METHODS: Concentric needle EMG signals were recorded from the tibialis anterior of healthy subjects as force was gradually increased from 0 to maximum force. The iEMG filling process was analyzed by measuring the EMG filling factor (FF), calculated from the mean rectified iEMG and the root mean square iEMG. RESULTS: (1) The iEMG activity at low contraction forces was "discrete" (FF<
0.3) for all participants. (2) The iEMG activity at maximal effort was "full" (FF>
0.5) for 83 % of the participants, whereas it was "incompletely-reduced" (0.3<
FF<
0.5) for 17 % of the participants. (3) The FF increased rapidly for forces up to 20 % MVC, and then levelled off for higher forces: thus, the FF curve had a typical exponential shape. CONCLUSIONS: The iEMG filling method can be considered of general applicability since the FF increased over a wide range in all healthy participants. SIGNIFICANCE: The EMG filling analysis may have potential to detect scenarios of MU loss and remodelling in neurogenic and motor neuron diseases.