Khảo sát khả năng kháng ấu trùng gây bệnh từ muỗi vằn và kháng vi sinh vật kiểm định của cao chiết cây lá đắng (Vernonia amygdalina Del.)=Evaluating the larvicidal and antimicrobial potential of Vernonia amygdalina (Bitter Leaf) extract against Aedes Mosquito Larvae and pathogenic microorganisms

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Tác giả: Thái Lan Anh Nguyễn, Thị Bảo Vy Nguyễn, Thị Ngọc Tuyên Nguyễn, Văn Khang Hy Nguyễn, Nữ Liên Hương Tôn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Công thương, 2024

Mô tả vật lý: tr.381-388

Bộ sưu tập: Metadata

ID: 475207

Thêm vào giỏ

Vernonia amygdaỉina Del., thuộc họ Asteraceae là loại cây mọc tự nhiên được tìm thấy ở nhiều nơi trên thế giới, đặc biệt thích hợp với khí hậu nóng ẩm như ở châu Phi và các nước Đông Nam Á. Trong số các hoạt tính sinh học đa dạng của cây lá đắng, do rất nhiều loại hợp chất thành phần, chúng tôi quan tâm nghiên cứu khả năng diệt bọ gậy muỗi của cây diệt ký sinh trùng này. Nguyên liệu được sử dụng trong nghiên cứu này là bột cây thu hái tại Cần Thơ, được ngâm chiết bằng methanol để thu dịch alcol tổng. Dịch alcol tiếp theo được chiết phân bố lần lượt với dung môi hữu cơ là n-hexane và ethyl acetate để tạo ra các dịch chiết có độ phân cực khác nhau. Trước tiên, thử nghiệm Brine-shrimp với các mẫu chiết xuất, tức là khảo sát khả năng gây độc của chiết xuất trên các sinh vật nhỏ như ấu trùng Artemia salina. Hai trong số 4 dịch chiết của V. amygdalina cho kết quả tốt về độc tính đối với ấu trùng Artemia salina là dịch chiết xuất MeOH tổng và EA. Hai hỗn hợp chiết xuất này thể hiện giá trị LC 50 tốt hơn một số chất tiêu chuẩn được sử dụng trong phương pháp Meyer. Tiếp theo, khảo sát khả năng diệt ấu trùng gây bệnh của muỗi Aedes qua việc diệt bọ gậy, trong các mẫu thì dịch chiết hexane thể hiện khả năng gây độc tốt nhất. Ngoài ra, khảo sát kháng khuẩn cho thấy các chiết xuất của cây lá đắng này chỉ ức chế yếu các vi sinh vật kiểm định gồm khuẩn Gram âm, Gram dương và nấm men ở mức nồng độ mg/mL.Vernonia amygdalina Del., a member of the Asteraceae family, thrives in hot, humid climates like those of Africa and Southeast Asia. Among its diverse biological activities, this study focused on the mosquito larvicidal properties of V. amygdalina. Plant powder collected in Can Tho was extracted with methanol to produce an alcohol extract, which was then sequentially partitioned using n-hexane and ethyl acetate to obtain fractions of varying polarities. Initial toxicity screening was conducted using the brine shrimp lethality assay, which revealed promising toxicity in two extracts - the total methanol (MeOH) and ethyl acetate (EA) fractions - showing superior LC 50 values compared to some standards in the Meyer method. Subsequent testing for larvicidal activity against Aedes mosquito larvae demonstrated that the hexane extract exhibited the highest toxicity. An antibacterial assay, however, indicated that all extracts of V. amygdalina exhibited only weak inhibitory effects on the tested Gram-positive, Gram-negative bacteria, and yeast at mg/mL concentrations.

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