Khảo sát quá trình tạo màng cellulose vi khuẩn từ nguồn nguyên liệu bùn giãy=Surveying the formation process of bacterial cellulose membrane from paper sludge

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Tác giả: Thị Tuyết Phạm, Bội An Trần, Thị Tưởng An Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Công thương, 2024

Mô tả vật lý: tr.389-396

Bộ sưu tập: Metadata

ID: 475208

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Bacterial cellulose (BC) membranes, valued for their purity due to the absence of lignin and hemicellulose typical of plant-derived cellulose, hold significant potential across scientific and technological applications due to their eco-friendliness, rapid biomass growth, and advantageous mechanical and physical properties. However, BC production depends on the availability of essential micronutrients for bacterial growth. This study investigates optimized fermentation conditions for BC film synthesis from recycled paper sludge using Acetobacter xylinum. Paper sludge sourced from a paper mill was pre-washed, filtered, and weighed to determine dry mass before undergoing hydrolysis and fermentation. Results indicated that a 5% concentration of H 2 SO 4 for 72 hours was the optimal condition for hydrolyzing the sludge to prepare a suitable fermentation substrate. The fermentation process, carried out over 15 days with a microbiological inoculum concentration of 10% (v/v), maintained a pH of 4.5 and was supplemented with (NH 4 ) 2 SO 4 , (NH 4 ) 2 HPO 4 , and peptone. The resulting BC films were characterized by X-ray diffraction (XRD) for crystallinity, thermogravimetric analysis (TGA) for thermal decomposition, and Fourier-transform infrared spectroscopy (FTIR) for functional group analysis, demonstrating the effective transformation of paper sludge into high-quality BC.Cellulose vi khuẩn (BC) từ lâu đã được coi là một nguồn cellulose tinh khiết vì không có cấu trúc phức tạp với lignin và hemicellulose như cellulose thực vật (lignocellulose). BC là một vật liệu tiềm năng được sử dụng trong nhiều lĩnh vực khoa học và công nghệ khác nhau nhờ vào tính thân thiện với môi trường, tốc độ tăng trưởng sinh khối, và các tính chất cơ học và vật lý. Tuy nhiên, quy trình sản xuất BC yêu cầu tiêu thụ các vi chất dinh dưỡng cho vi khuẩn. Nghiên cứu này xác định và chọn lựa các điều kiện phù hợp cho quá trình lên men tạo cellulose từ bùn giấy tái chế với vi khuẩn Acetobacter xylinum. Bùn giấy từ nhà máy giấy đã được rửa, lọc, thủy phân và lên men. Kết quả của quá trình thủy phân bùn giấy ở các nồng độ H 2 SO 4 khác nhau (1-9%) trong 72 giờ. Kết quả cho thấy nồng độ 5% H 2 SO 4 là phù hợp. Thời gian lên men tối ưu để thu BC là 15 ngày, tỷ lệ bổ sung vi sinh 10% (v/v), pH của môi trường 4.5 với dinh dưỡng bổ sung là (NH 4 ) 2 SO 4 , (NH 4 ) 2 HPO 4 , peptone. Các tính chất của màng BC đã được phân tích, gồm độ tinh thể (XRD), nhiệt độ phân hủy (TGA), câu trúc của các nhóm chức hóa học (FTIR).

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